• Rat REN (Renin) CLIA Kit (STJC0001526)

Rat REN (Renin) CLIA Kit (STJC0001526)

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STJC0001526

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Applications: CLIA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This rat REN kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Ren1
Gene ID: 24715
Uniprot ID: RENI_RAT
Specificity: This kit recognizes Rat REN in samples. No significant cross-reactivity or interference between Rat REN and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Protein Name Renin
Angiotensinogenase
Database Links Reactome: R-RNO-2022377
Cellular Localisation Secreted
Membrane
Associated To Membranes Via Binding To Atp6ap2
Alternative CLIA Names Renin CLIA kit
Angiotensinogenase CLIA kit
Ren1 CLIA kit
Ren CLIA kit
Specificity This kit recognizes Rat REN in samples. No significant cross-reactivity or interference between Rat REN and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 98-110 103
EDTA plasma(n=8) 96-110 103
Cell culture media(n=8) 86-98 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 24.24 42.13 218.10 22.99 45.88 224.12
Standard deviation 1.99 3.30 23.93 2.40 4.81 18.15
CV (%) 8.21 7.83 10.97 10.44 10.48 8.10
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance