| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.069ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This Rac1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Rac1 |
| Gene ID: |
363875 |
| Uniprot ID: |
RAC1_RAT |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Osteoclasts. |
| Post Translational Modifications | GTP-bound active form is ubiquitinated by HACE1, leading to its degradation by the proteasome. Phosphorylated by AKT at Ser-71. Ubiquitinated at Lys-166 in a FBXL19-mediated manner.leading to proteasomal degradation. |
| Function | Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2.this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In neurons, is involved in dendritic spine formation and synaptic plasticity. In hippocampal neurons, involved in spine morphogenesis and synapse formation, through local activation at synapses by guanine nucleotide exchange factors (GEFs), such as ARHGEF6/ARHGEF7/PIX. In synapses, may mediate the regulation of F-actin cluster formation performed by SHANK3. In neurons, plays a crucial role in regulating GABA(A) receptor synaptic stability and hence GABAergic inhibitory synaptic transmission through its role in PAK1 activation and eventually F-actin stabilization. |
| Protein Name | Ras-Related C3 Botulinum Toxin Substrate 1P21-Rac1 |
| Database Links | Reactome: R-RNO-114604Reactome: -RNO-1257604Reactome: -RNO-1433557Reactome: -RNO-193648Reactome: -RNO-2029482Reactome: -RNO-2424491Reactome: -RNO-2871796Reactome: -RNO-376172Reactome: -RNO-389359Reactome: -RNO-3928662Reactome: -RNO-3928664Reactome: -RNO-3928665Reactome: -RNO-399954Reactome: -RNO-4086400Reactome: -RNO-416550Reactome: -RNO-418885Reactome: -RNO-4420097Reactome: -RNO-445144Reactome: -RNO-5218920Reactome: -RNO-5625740Reactome: -RNO-5625900Reactome: -RNO-5625970Reactome: -RNO-5626467Reactome: -RNO-5627123Reactome: -RNO-5663213Reactome: -RNO-5663220Reactome: -RNO-5668599Reactome: -RNO-5687128Reactome: -RNO-6798695Reactome: -RNO-6811558Reactome: -RNO-8849471Reactome: -RNO-8875555Reactome: -RNO-9013149Reactome: -RNO-9032759Reactome: -RNO-9748787Reactome: -RNO-983231 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideMelanosomeCytoplasmCell ProjectionLamellipodiumDendriteSynapseNucleusInner Surface Of Plasma Membrane Possibly With Attachment Requiring Prenylation Of The C-Terminal CysteineFound In The Ruffled Border (A Late Endosomal-Like Compartment In The Plasma Membrane) Of Bone-Resorbing OsteoclastsLocalizes To The Lamellipodium In A Sh3rf1-Dependent MannerIn MacrophagesCytoplasmic Location Increases Upon Csf1 StimulationIn Hippocampal NeuronsLocalizes At SynapsesWhere It Is ActivatedActivation By Gtp-Binding Promotes Nuclear Localization |
| Alternative ELISA Names | Ras-Related C3 Botulinum Toxin Substrate 1 ELISA kitP21-Rac1 ELISA kitRac1 ELISA kitRac ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
