• Rat Rac1 (Ras Related C3 Botulinum Toxin Substrate 1) Sandwich ELISA Kit Ready-To-Use (STJE0018194)

Rat Rac1 (Ras Related C3 Botulinum Toxin Substrate 1) Sandwich ELISA Kit Ready-To-Use (STJE0018194)

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STJE0018194

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Applications: ELISA
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.069ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This Rac1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: Rac1
Gene ID: 363875
Uniprot ID: RAC1_RAT
Immunogen Region: Ready-To-Use
Sample Type: tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity Osteoclasts.
Post Translational Modifications GTP-bound active form is ubiquitinated by HACE1, leading to its degradation by the proteasome. Phosphorylated by AKT at Ser-71. Ubiquitinated at Lys-166 in a FBXL19-mediated manner.leading to proteasomal degradation.
Function Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2.this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In neurons, is involved in dendritic spine formation and synaptic plasticity. In hippocampal neurons, involved in spine morphogenesis and synapse formation, through local activation at synapses by guanine nucleotide exchange factors (GEFs), such as ARHGEF6/ARHGEF7/PIX. In synapses, may mediate the regulation of F-actin cluster formation performed by SHANK3. In neurons, plays a crucial role in regulating GABA(A) receptor synaptic stability and hence GABAergic inhibitory synaptic transmission through its role in PAK1 activation and eventually F-actin stabilization.
Protein Name Ras-Related C3 Botulinum Toxin Substrate 1
P21-Rac1
Database Links Reactome: R-RNO-114604
Reactome: -RNO-1257604
Reactome: -RNO-1433557
Reactome: -RNO-193648
Reactome: -RNO-2029482
Reactome: -RNO-2424491
Reactome: -RNO-2871796
Reactome: -RNO-376172
Reactome: -RNO-389359
Reactome: -RNO-3928662
Reactome: -RNO-3928664
Reactome: -RNO-3928665
Reactome: -RNO-399954
Reactome: -RNO-4086400
Reactome: -RNO-416550
Reactome: -RNO-418885
Reactome: -RNO-4420097
Reactome: -RNO-445144
Reactome: -RNO-5218920
Reactome: -RNO-5625740
Reactome: -RNO-5625900
Reactome: -RNO-5625970
Reactome: -RNO-5626467
Reactome: -RNO-5627123
Reactome: -RNO-5663213
Reactome: -RNO-5663220
Reactome: -RNO-5668599
Reactome: -RNO-5687128
Reactome: -RNO-6798695
Reactome: -RNO-6811558
Reactome: -RNO-8849471
Reactome: -RNO-8875555
Reactome: -RNO-9013149
Reactome: -RNO-9032759
Reactome: -RNO-9748787
Reactome: -RNO-983231
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Melanosome
Cytoplasm
Cell Projection
Lamellipodium
Dendrite
Synapse
Nucleus
Inner Surface Of Plasma Membrane Possibly With Attachment Requiring Prenylation Of The C-Terminal Cysteine
Found In The Ruffled Border (A Late Endosomal-Like Compartment In The Plasma Membrane) Of Bone-Resorbing Osteoclasts
Localizes To The Lamellipodium In A Sh3rf1-Dependent Manner
In Macrophages
Cytoplasmic Location Increases Upon Csf1 Stimulation
In Hippocampal Neurons
Localizes At Synapses
Where It Is Activated
Activation By Gtp-Binding Promotes Nuclear Localization
Alternative ELISA Names Ras-Related C3 Botulinum Toxin Substrate 1 ELISA kit
P21-Rac1 ELISA kit
Rac1 ELISA kit
Rac ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance