| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This rat PKR1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Prokr1 |
| Gene ID: |
192648 |
| Uniprot ID: |
PKR1_RAT |
| Specificity: |
This kit recognizes Rat PKR1 in samples. No significant cross-reactivity or interference between Rat PKR1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Widely expressed in peripheral tissues with the highest level in the spleen and moderate levels in the adipose tissues, thymus, lung, kidney, testis, uterus and small intestine. |
| Function | Receptor for prokineticin 1. Exclusively coupled to the G(q) subclass of heteromeric G proteins. Activation leads to mobilization of calcium, stimulation of phosphoinositide turnover and activation of p44/p42 mitogen-activated protein kinase. May play a role during early pregnancy. |
| Protein Name | Prokineticin Receptor 1Pk-R1G-Protein Coupled Receptor 73G-Protein Coupled Receptor Zaq |
| Database Links | Reactome: R-RNO-375276Reactome: -RNO-416476 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative CLIA Names | Prokineticin Receptor 1 CLIA kitPk-R1 CLIA kitG-Protein Coupled Receptor 73 CLIA kitG-Protein Coupled Receptor Zaq CLIA kitProkr1 CLIA kitGpr73 CLIA kitPkr1 CLIA kit |
| Specificity | This kit recognizes Rat PKR1 in samples. No significant cross-reactivity or interference between Rat PKR1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 99-115 | 105 |
| EDTA plasma(n=8) | 90-103 | 96 |
| Cell culture media(n=8) | 100-116 | 107 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 95.51 | 279.05 | 755.17 | 98.11 | 270.15 | 770.49 |
| Standard deviation | 7.76 | 29.24 | 46.37 | 10.57 | 29.55 | 76.74 |
| CV (%) | 8.12 | 10.48 | 6.14 | 10.77 | 10.94 | 9.96 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
