• Rat PCNA (Proliferating Cell Nuclear Antigen) CLIA Kit (STJC0001859)

Rat PCNA (Proliferating Cell Nuclear Antigen) CLIA Kit (STJC0001859)

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STJC0001859

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Applications: CLIA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This rat PCNA kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Pcna
Gene ID: 25737
Uniprot ID: PCNA_RAT
Specificity: This kit recognizes Rat PCNA in samples. No significant cross-reactivity or interference between Rat PCNA and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Phosphorylated. Phosphorylation at Tyr-211 by EGFR stabilizes chromatin-associated PCNA. Acetylated by CREBBP and p300/EP300.preferentially acetylated by CREBBP on Lys-80, Lys-13 and Lys-14 and on Lys-77 by p300/EP300 upon loading on chromatin in response to UV irradiation. Lysine acetylation disrupts association with chromatin, hence promoting PCNA ubiquitination and proteasomal degradation in response to UV damage in a CREBBP- and EP300-dependent manner. Acetylation disrupts interaction with NUDT15 and promotes degradation. Ubiquitinated. Following DNA damage, can be either monoubiquitinated to stimulate direct bypass of DNA lesions by specialized DNA polymerases or polyubiquitinated to promote recombination-dependent DNA synthesis across DNA lesions by template switching mechanisms. Following induction of replication stress, monoubiquitinated by the UBE2B-RAD18 complex on Lys-164, leading to recruit translesion (TLS) polymerases, which are able to synthesize across DNA lesions in a potentially error-prone manner. An error-free pathway also exists and requires non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH. This error-free pathway, also known as template switching, employs recombination mechanisms to synthesize across the lesion, using as a template the undamaged, newly synthesized strand of the sister chromatid. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis. Sumoylated during S phase. Methylated on glutamate residues by ARMT1.
Function Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Protein Name Proliferating Cell Nuclear Antigen
Pcna
Cyclin
Database Links Reactome: R-RNO-110312
Reactome: -RNO-110314
Reactome: -RNO-110320
Reactome: -RNO-174411
Reactome: -RNO-174414
Reactome: -RNO-174417
Reactome: -RNO-174437
Reactome: -RNO-4615885
Reactome: -RNO-5358565
Reactome: -RNO-5651801
Reactome: -RNO-5655862
Reactome: -RNO-5656121
Reactome: -RNO-5656169
Reactome: -RNO-5685942
Reactome: -RNO-5696397
Reactome: -RNO-5696400
Reactome: -RNO-6782135
Reactome: -RNO-6782210
Reactome: -RNO-6804114
Reactome: -RNO-69091
Reactome: -RNO-69166
Reactome: -RNO-69183
Reactome: -RNO-8866654
Cellular Localisation Nucleus
Forms Nuclear Foci Representing Sites Of Ongoing Dna Replication And Vary In Morphology And Number During S Phase
Together With Apex2
Is Redistributed In Discrete Nuclear Foci In Presence Of Oxidative Dna Damaging Agents
Colocalizes With Crebbp
Ep300 And Pold1 To Sites Of Dna Damage
Alternative CLIA Names Proliferating Cell Nuclear Antigen CLIA kit
Pcna CLIA kit
Cyclin CLIA kit
Pcna CLIA kit
Specificity This kit recognizes Rat PCNA in samples. No significant cross-reactivity or interference between Rat PCNA and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-107 99
EDTA plasma(n=8) 84-98 91
Cell culture media(n=8) 94-106 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 97.12 194.96 724.41 90.17 208.15 665.47
Standard deviation 11.97 19.81 61.21 11.48 23.50 55.37
CV (%) 12.32 10.16 8.45 12.73 11.29 8.32
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance