Rat NGAL (NeutrophilGelatinase Associated Lipocalin) Sandwich ELISA (STJE0019945)

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STJE0019945

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Applications: ELISA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.84 pg/mL
Detection Limit: 1.56-100 pg/mL
Short Description: This NGAL Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Lcn2
Gene ID: 170496
Uniprot ID: NGAL_RAT
Specificity: This kit recognizes Rat NGAL in samples. No significant cross-reactivity or interference between Rat NGAL and analogues was observed.
Sample Type: serum, plasma and other biological fluids
Tissue Specificity Detected in the ureteric bud in embryonic kidney (at protein level).
Function Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity.limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin. Can also bind siderophores from M.tuberculosis.
Protein Name Neutrophil Gelatinase-Associated Lipocalin
Ngal
Alpha-2-Microglobulin-Related Protein
Alpha-2u Globulin-Related Protein
Lipocalin 24p3
Lipocalin-2
Siderocalin Lcn2
P25
Database Links Reactome: R-RNO-6798695
Reactome: -RNO-6799990
Reactome: -RNO-917937
Cellular Localisation Secreted
Cytoplasmic Granule Lumen
Cytoplasmic Vesicle Lumen
Upon Binding To The Slc22a17 (24p3r) Receptor
It Is Internalized
Releases The Bound Iron In The Acidic Lumen Of Cytoplasmic Vesicles
Alternative ELISA Names Neutrophil Gelatinase-Associated Lipocalin ELISA kit
Ngal ELISA kit
Alpha-2-Microglobulin-Related Protein ELISA kit
Alpha-2u Globulin-Related Protein ELISA kit
Lipocalin 24p3 ELISA kit
Lipocalin-2 ELISA kit
Siderocalin Lcn2 ELISA kit
P25 ELISA kit
Lcn2 ELISA kit
Specificity This kit recognizes Rat NGAL in samples. No significant cross-reactivity or interference between Rat NGAL and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-104 99
EDTA plasma (n=8) 94-111 102
Cell culture media (n=8) 85-101 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 5.26 11.69 48.76 5.01 11.83 50.70
Standard deviation 0.29 0.56 2.07 0.28 0.64 1.97
CV (%) 5.49 4.82 4.25 5.49 5.39 3.88
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance