| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
13.3pg/mL |
| Detection Limit: |
31.2-2000pg/mL |
| Short Description: |
This NEP Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Mme |
| Gene ID: |
24590 |
| Uniprot ID: |
NEP_RAT |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Myristoylation is a determinant of membrane targeting. Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity. |
| Function | Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Catalyzes cleavage of bradykinin, substance P and neurotensin peptides. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of the atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers. |
| Protein Name | NeprilysinAtriopeptidaseEnkephalinaseNeutral Endopeptidase 24.11NepNeutral EndopeptidaseSkin Fibroblast ElastaseSfeCd Antigen Cd10 |
| Database Links | Reactome: R-RNO-2022377Reactome: -RNO-5578768Reactome: -RNO-6798695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane Protein |
| Alternative ELISA Names | Neprilysin ELISA kitAtriopeptidase ELISA kitEnkephalinase ELISA kitNeutral Endopeptidase 24.11 ELISA kitNep ELISA kitNeutral Endopeptidase ELISA kitSkin Fibroblast Elastase ELISA kitSfe ELISA kitCd Antigen Cd10 ELISA kitMme ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
