| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.108ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This IREB2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Ireb2 |
| Gene ID: |
64831 |
| Uniprot ID: |
IREB2_RAT |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Ubiquitously expressed in rat tissues, the highest amounts present in skeletal muscle and heart. |
| Post Translational Modifications | Ubiquitinated and degraded by the proteasome in presence of high level of iron and oxygen. Ubiquitinated by a SCF complex containing FBXL5. Upon iron and oxygen depletion FBXL5 is degraded, preventing ubiquitination and allowing its RNA-binding activity. |
| Function | RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. |
| Protein Name | Iron-Responsive Element-Binding Protein 2Ire-Bp 2Iron Regulatory Protein 2Irp2 |
| Database Links | Reactome: R-RNO-917937 |
| Cellular Localisation | Cytoplasm |
| Alternative ELISA Names | Iron-Responsive Element-Binding Protein 2 ELISA kitIre-Bp 2 ELISA kitIron Regulatory Protein 2 ELISA kitIrp2 ELISA kitIreb2 ELISA kitIrp2 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
