| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.103ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This HSPA8 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Hspa8 |
| Gene ID: |
24468 |
| Uniprot ID: |
HSP7C_RAT |
| Sample Type: |
serum, plasma or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Acetylated. ISGylated. Trimethylation at Lys-561 reduces fibrillar SNCA binding. |
| Function | Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, chaperone-mediated autophagy, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The co-chaperones have been shown to not only regulate different steps of the ATPase cycle of HSP70, but they also have an individual specificity such that one co-chaperone may promote folding of a substrate while another may promote degradation. The affinity of HSP70 for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. HSP70 goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. The HSP70-associated co-chaperones are of three types: J-domain co-chaperones HSP40s (stimulate ATPase hydrolysis by HSP70), the nucleotide exchange factors (NEF) such as BAG1/2/3 (facilitate conversion of HSP70 from the ADP-bound to the ATP-bound state thereby promoting substrate release), and the TPR domain chaperones such as HOPX and STUB1. Plays a critical role in mitochondrial import, delivers preproteins to the mitochondrial import receptor TOMM70. Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Substrate recognition component in chaperone-mediated autophagy (CMA), a selective protein degradation process that mediates degradation of proteins with a -KFERQ motif: HSPA8/HSC70 specifically recognizes and binds cytosolic proteins bearing a -KFERQ motif and promotes their recruitment to the surface of the lysosome where they bind to lysosomal protein LAMP2. KFERQ motif-containing proteins are eventually transported into the lysosomal lumen where they are degraded. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase STUB1. Interacts with VGF-derived peptide TLQP-21. |
| Protein Name | Heat Shock Cognate 71 Kda ProteinHeat Shock 70 Kda Protein 8 |
| Database Links | Reactome: R-RNO-3371453Reactome: -RNO-3371497Reactome: -RNO-3371568Reactome: -RNO-3371571Reactome: -RNO-432720Reactome: -RNO-432722Reactome: -RNO-450408Reactome: -RNO-6798695Reactome: -RNO-72163Reactome: -RNO-8856828Reactome: -RNO-8876725Reactome: -RNO-888590 |
| Cellular Localisation | CytoplasmMelanosomeNucleusNucleolusCell MembraneLysosome MembranePeripheral Membrane ProteinCytoplasmic SideLocalized In Cytoplasmic Mrnp Granules Containing Untranslated MrnasTranslocates Rapidly From The Cytoplasm To The NucleiAnd Especially To The NucleoliUpon Heat Shock |
| Alternative ELISA Names | Heat Shock Cognate 71 Kda Protein ELISA kitHeat Shock 70 Kda Protein 8 ELISA kitHspa8 ELISA kitHsc70 ELISA kitHsc73 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
