| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
25pg/mL |
| Detection Limit: |
78.1-5000pg/mL |
| Short Description: |
This HIF2a Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Epas1 |
| Gene ID: |
29452 |
| Uniprot ID: |
EPAS1_RAT |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | In normoxia, is probably hydroxylated on Pro-405 and Pro-530 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization. In normoxia, is hydroxylated on Asn-851 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. Phosphorylated on multiple sites in the CTAD. The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains. |
| Function | Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT.heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. |
| Protein Name | Endothelial Pas Domain-Containing Protein 1Epas-1Hypoxia-Inducible Factor 2-AlphaHif-2-AlphaHif2-Alpha |
| Database Links | Reactome: R-RNO-1234158Reactome: -RNO-1234174Reactome: -RNO-1234176Reactome: -RNO-8951664 |
| Cellular Localisation | NucleusNucleus SpeckleColocalizes With Hif3a In The Nucleus And Speckles |
| Alternative ELISA Names | Endothelial Pas Domain-Containing Protein 1 ELISA kitEpas-1 ELISA kitHypoxia-Inducible Factor 2-Alpha ELISA kitHif-2-Alpha ELISA kitHif2-Alpha ELISA kitEpas1 ELISA kitHif2a ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
