| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
1.88ng/mL |
| Detection Limit: |
3.13~200ng/mL |
| Short Description: |
This rat GS kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Gsn |
| Gene ID: |
296654 |
| Uniprot ID: |
GELS_RAT |
| Specificity: |
This kit recognizes Rat GS in samples. No significant cross-reactivity or interference between Rat GS and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylated on tyrosine residues in vitro. |
| Function | Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis. |
| Protein Name | GelsolinActin-Depolymerizing FactorAdfBrevin |
| Database Links | Reactome: R-RNO-264870Reactome: -RNO-6798695 |
| Cellular Localisation | Isoform 1: SecretedIsoform 2: CytoplasmCytoskeletonA Cytoplasmic Form May Also Exist |
| Alternative CLIA Names | Gelsolin CLIA kitActin-Depolymerizing Factor CLIA kitAdf CLIA kitBrevin CLIA kitGsn CLIA kit |
| Specificity | This kit recognizes Rat GS in samples. No significant cross-reactivity or interference between Rat GS and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-106 | 97 |
| EDTA plasma(n=8) | 89-101 | 95 |
| Cell culture media(n=8) | 93-105 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 9.72 | 23.08 | 76.59 | 9.26 | 22.86 | 72.33 |
| Standard deviation | 1.26 | 1.70 | 6.41 | 1.10 | 2.71 | 6.94 |
| CV (%) | 12.96 | 7.37 | 8.37 | 11.88 | 11.85 | 9.59 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
