| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.47ng/mL |
| Detection Limit: |
0.78~50ng/mL |
| Short Description: |
This Got2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Got2 |
| Gene ID: |
25721 |
| Uniprot ID: |
AATM_RAT |
| Specificity: |
This kit recognizes Rat FABP1 in samples. No significant cross-reactivity or interference between Rat FABP1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids. |
| Protein Name | Aspartate Aminotransferase - MitochondrialMaspatFatty Acid-Binding ProteinFabp-1Glutamate Oxaloacetate Transaminase 2Kynurenine Aminotransferase 4Kynurenine Aminotransferase IvKynurenine--Oxoglutarate Transaminase 4Kynurenine--Oxoglutarate Transaminase IvPlasma Membrane-Associated Fatty Acid-Binding ProteinFabppmTransaminase A |
| Database Links | Reactome: R-RNO-389661Reactome: -RNO-70263Reactome: -RNO-8963693Reactome: -RNO-8964539 |
| Cellular Localisation | Mitochondrion MatrixCell MembraneLocated In The Mitochondria Of LiverPancreasSpleenHeartPituitary Gland And Submandibular Gland CellsIn KidneyLocated In The MitochondriaOn The Cell Surface Of Regions With Protrusions In Distal TubulesOn The Apical Cell Surface Of Protrusions Along The Microvilli In Cortical Collecting DuctsIn Condensing VacuolesOn The Cell Surface At Cell Boundaries Of Adjoining Kidney Cells And On The Cell Surface Of Endothelial Cells Lining Capillaries In The GlomerulusAlso Located At The Cell Surface Of Endothelial Cells Lining Arterioles And On The Cell Surface Of Lymphocytes |
| Alternative ELISA Names | Aspartate Aminotransferase - Mitochondrial ELISA kitMaspat ELISA kitFatty Acid-Binding Protein ELISA kitFabp-1 ELISA kitGlutamate Oxaloacetate Transaminase 2 ELISA kitKynurenine Aminotransferase 4 ELISA kitKynurenine Aminotransferase Iv ELISA kitKynurenine--Oxoglutarate Transaminase 4 ELISA kitKynurenine--Oxoglutarate Transaminase Iv ELISA kitPlasma Membrane-Associated Fatty Acid-Binding Protein ELISA kitFabppm ELISA kitTransaminase A ELISA kitGot2 ELISA kitMaat ELISA kit |
| Specificity | This kit recognizes Rat FABP1 in samples. No significant cross-reactivity or interference between Rat FABP1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 97-110 | 103 |
| EDTA plasma (n=8) | 84-97 | 92 |
| Cell culture media (n=8) | 84-93 | 90 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.06 | 5.63 | 22.21 | 2.04 | 5.36 | 22.54 |
| Standard deviation | 0.10 | 0.26 | 1.12 | 0.12 | 0.26 | 1.17 |
| CV (%) | 4.85 | 4.62 | 5.04 | 5.88 | 4.85 | 5.19 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
