| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.94ng/mL |
| Detection Limit: |
1.56~100ng/mL |
| Short Description: |
This Ggt1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Ggt1 |
| Gene ID: |
116568 |
| Uniprot ID: |
GGT1_RAT |
| Specificity: |
This kit recognizes Rat Gamma GT1 in samples. No significant cross-reactivity or interference between Rat Gamma GT1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | N-glycosylated on both chains.contains sialic acid residues. It is not known if the sialic acid residues are present on N-linked or on O-linked glycans. O-glycosylated.close to the membrane anchor on the heavy chain and on the light chain. The sugar moieties are localized to the stretch Thr-28 to Ser-30. Contains sialic acid residues. It is not known if the sialic acid residues are present on N-linked or on O-linked glycans. Cleaved by autocatalysis into a large and a small subunit and the autocatalytic cleavage is essential to the functional activation of the enzyme. |
| Function | Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates (such as maresin conjugate (13R)-S-glutathionyl-(14S)-hydroxy-(4Z,7Z,9E,11E,16Z,19Z)-docosahexaenoate, MCTR1) and other gamma-glutamyl compounds (such as leukotriene C4, LTC4). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4. |
| Protein Name | Glutathione Hydrolase 1 ProenzymeGamma-Glutamyltransferase 1Gamma-Glutamyltranspeptidase 1Ggt 1Leukotriene-C4 HydrolaseCd Antigen Cd224 Cleaved Into - Glutathione Hydrolase 1 Heavy Chain - Glutathione Hydrolase 1 Light Chain |
| Database Links | Reactome: R-RNO-174403Reactome: -RNO-2142691Reactome: -RNO-5423646Reactome: -RNO-9753281 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane Protein |
| Alternative ELISA Names | Glutathione Hydrolase 1 Proenzyme ELISA kitGamma-Glutamyltransferase 1 ELISA kitGamma-Glutamyltranspeptidase 1 ELISA kitGgt 1 ELISA kitLeukotriene-C4 Hydrolase ELISA kitCd Antigen Cd224 Cleaved Into - Glutathione Hydrolase 1 Heavy Chain - Glutathione Hydrolase 1 Light Chain ELISA kitGgt1 ELISA kitGgt ELISA kit |
| Specificity | This kit recognizes Rat γGT1 in samples. No significant cross-reactivity or interference between Rat γGT1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-101 | 93 |
| EDTA plasma (n=8) | 90-103 | 95 |
| Cell culture media (n=8) | 90-104 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 4.81 | 9.78 | 41.87 | 4.36 | 9.54 | 44.98 |
| Standard deviation | 0.30 | 0.48 | 1.98 | 0.28 | 0.39 | 1.41 |
| CV (%) | 6.24 | 4.91 | 4.73 | 6.42 | 4.09 | 3.13 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
