| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This GFRa1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Gfra1 |
| Gene ID: |
25454 |
| Uniprot ID: |
GFRA1_RAT |
| Specificity: |
This kit recognizes Rat GFRa1 in samples. No significant cross-reactivity or interference between Rat GFRa1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in liver, brain, kidney and cochlea. |
| Function | Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor. |
| Protein Name | Gdnf Family Receptor Alpha-1Gdnf Receptor Alpha-1Gdnfr-Alpha-1Gfr-Alpha-1Ret Ligand 1Tgf-Beta-Related Neurotrophic Factor Receptor 1 |
| Database Links | Reactome: R-RNO-5673001Reactome: -RNO-8853659 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorGolgi ApparatusTrans-Golgi NetworkEndosomeMultivesicular BodyLocalizes Mainly To The Plasma MembraneIn The Presence Of Sorl1Shifts To Vesicular StructuresIncluding Trans-Golgi NetworkEndosomes And Multivesicular Bodies |
| Alternative ELISA Names | Gdnf Family Receptor Alpha-1 ELISA kitGdnf Receptor Alpha-1 ELISA kitGdnfr-Alpha-1 ELISA kitGfr-Alpha-1 ELISA kitRet Ligand 1 ELISA kitTgf-Beta-Related Neurotrophic Factor Receptor 1 ELISA kitGfra1 ELISA kitGdnfra ELISA kitRetl1 ELISA kitTrnr1 ELISA kit |
| Specificity | This kit recognizes Rat GFRa1 in samples. No significant cross-reactivity or interference between Rat GFRa1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-104 | 97 |
| EDTA plasma (n=8) | 88-102 | 95 |
| Cell culture media (n=8) | 87-101 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 98.05 | 289.24 | 787.56 | 89.47 | 277.95 | 721.80 |
| Standard deviation | 5.56 | 15.30 | 41.35 | 5.97 | 13.87 | 35.15 |
| CV (%) | 5.67 | 5.29 | 5.25 | 6.67 | 4.99 | 4.87 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
