Rat GFRa1 (Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1) Sandwich ELISA (STJE0019787)

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STJE0019787

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Applications: ELISA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This GFRa1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Gfra1
Gene ID: 25454
Uniprot ID: GFRA1_RAT
Specificity: This kit recognizes Rat GFRa1 in samples. No significant cross-reactivity or interference between Rat GFRa1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in liver, brain, kidney and cochlea.
Function Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor.
Protein Name Gdnf Family Receptor Alpha-1
Gdnf Receptor Alpha-1
Gdnfr-Alpha-1
Gfr-Alpha-1
Ret Ligand 1
Tgf-Beta-Related Neurotrophic Factor Receptor 1
Database Links Reactome: R-RNO-5673001
Reactome: -RNO-8853659
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Golgi Apparatus
Trans-Golgi Network
Endosome
Multivesicular Body
Localizes Mainly To The Plasma Membrane
In The Presence Of Sorl1
Shifts To Vesicular Structures
Including Trans-Golgi Network
Endosomes And Multivesicular Bodies
Alternative ELISA Names Gdnf Family Receptor Alpha-1 ELISA kit
Gdnf Receptor Alpha-1 ELISA kit
Gdnfr-Alpha-1 ELISA kit
Gfr-Alpha-1 ELISA kit
Ret Ligand 1 ELISA kit
Tgf-Beta-Related Neurotrophic Factor Receptor 1 ELISA kit
Gfra1 ELISA kit
Gdnfra ELISA kit
Retl1 ELISA kit
Trnr1 ELISA kit
Specificity This kit recognizes Rat GFRa1 in samples. No significant cross-reactivity or interference between Rat GFRa1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-104 97
EDTA plasma (n=8) 88-102 95
Cell culture media (n=8) 87-101 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 98.05 289.24 787.56 89.47 277.95 721.80
Standard deviation 5.56 15.30 41.35 5.97 13.87 35.15
CV (%) 5.67 5.29 5.25 6.67 4.99 4.87
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance