| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This GC Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
2.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
GcgUniprot ID=GLUC_RAT" |
| Specificity: |
This kit recognizes Rat GC in samples. No significant cross-reactivity or interference between Rat GC and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas. |
| Function | Glucagon: Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon-like peptide 1: Potent stimulator of glucose-dependent insulin release (Probable). Also stimulates insulin release in response to IL6. Plays important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Has growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation. Inhibits beta cell apoptosis (Probable). Glucagon-like peptide 2: Stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability. Glicentin: May modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life. Oxyntomodulin significantly reduces food intake. |
| Protein Name | Pro-Glucagon Cleaved Into - Glicentin - Glicentin-Related PolypeptideGrpp - OxyntomodulinOxmOxy - Glucagon - Glucagon-Like Peptide 1Glp-1 - Glucagon-Like Peptide 1(7-37Glp-1(7-37 - Glucagon-Like Peptide 1(7-36Glp-1(7-36 - Glucagon-Like Peptide 2Glp-2 |
| Cellular Localisation | SecretedGlucagon-Like Peptide 1: Secreted |
| Alternative ELISA Names | Pro-Glucagon Cleaved Into - Glicentin - Glicentin-Related Polypeptide ELISA kitGrpp - Oxyntomodulin ELISA kitOxm ELISA kitOxy - Glucagon - Glucagon-Like Peptide 1 ELISA kitGlp-1 - Glucagon-Like Peptide 1(7-37 ELISA kitGlp-1(7-37 - Glucagon-Like Peptide 1(7-36 ELISA kitGlp-1(7-36 - Glucagon-Like Peptide 2 ELISA kitGlp-2 ELISA kitGcg ELISA kit |
| Specificity | This kit recognizes GC in samples. No significant cross-reactivity or interference between GC and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8℃ |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-107 | 98 |
| EDTA plasma(n=8) | 92-104 | 98 |
| Cell culture media(n=8) | 95-106 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 205.30 | 401.50 | 1351.70 | 185.10 | 391.80 | 1483.10 |
| Standard deviation | 11.70 | 17.30 | 58.10 | 9.80 | 18.00 | 60.80 |
| CV (%) | 5.70 | 4.31 | 4.30 | 5.29 | 4.59 | 4.10 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
