| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This rat FRS3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Frs3 |
| Gene ID: |
316213 |
| Uniprot ID: |
FRS3_RAT |
| Specificity: |
This kit recognizes Rat FRS3 in samples. No significant cross-reactivity or interference between Rat FRS3 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylated on tyrosine residues upon stimulation by BFGF or NGFB. |
| Function | Adapter protein that links FGF and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Down-regulates ERK2 signaling by interfering with the phosphorylation and nuclear translocation of ERK2. |
| Protein Name | Fibroblast Growth Factor Receptor Substrate 3Fgfr Substrate 3Fgfr-Signaling Adaptor Snt2Suc1-Associated Neurotrophic Factor Target 2Snt-2 |
| Database Links | Reactome: R-RNO-5654693Reactome: -RNO-5654700Reactome: -RNO-5654706Reactome: -RNO-5654712Reactome: -RNO-5673001Reactome: -RNO-9696270Reactome: -RNO-9696273 |
| Cellular Localisation | MembraneLipid-Anchor |
| Alternative CLIA Names | Fibroblast Growth Factor Receptor Substrate 3 CLIA kitFgfr Substrate 3 CLIA kitFgfr-Signaling Adaptor Snt2 CLIA kitSuc1-Associated Neurotrophic Factor Target 2 CLIA kitSnt-2 CLIA kitFrs3 CLIA kitSnt2 CLIA kit |
| Specificity | This kit recognizes Rat FRS3 in samples. No significant cross-reactivity or interference between Rat FRS3 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-102 | 97 |
| EDTA plasma(n=8) | 92-103 | 97 |
| Cell culture media(n=8) | 91-108 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 103.39 | 282.77 | 857.56 | 97.36 | 291.57 | 835.42 |
| Standard deviation | 8.74 | 30.54 | 71.18 | 7.99 | 20.50 | 80.28 |
| CV (%) | 8.45 | 10.80 | 8.30 | 8.21 | 7.03 | 9.61 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
