| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.127ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This FASN Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Fasn |
| Gene ID: |
50671 |
| Uniprot ID: |
FAS_RAT |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | S-nitrosylation of Fatty acid synthase at cysteine residues Cys-1464 or Cys-2085 is important for the enzyme dimerization. In adipocytes, S-nitrosylation of Fatty acid synthase occurs under physiological conditions and gradually increases during adipogenesis. |
| Function | Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein (ACP) domain. |
| Protein Name | Fatty Acid SynthaseType I Fas Includes - Acyl-Carrier-Protein S-Acetyltransferase - Acyl-Carrier-Protein S-Malonyltransferase - 3-Oxoacyl-Acyl-Carrier-Protein Synthase - 3-Oxoacyl-Acyl-Carrier-Protein Reductase - 3-Hydroxyacyl-Acyl-Carrier-Protein Dehydratase - Enoyl-Acyl-Carrier-Protein Reductase - Acyl-Acyl-Carrier-Protein Hydrolase |
| Database Links | Reactome: R-RNO-163765Reactome: -RNO-199220Reactome: -RNO-75105 |
| Cellular Localisation | CytoplasmMelanosome |
| Alternative ELISA Names | Fatty Acid Synthase ELISA kitType I Fas Includes - Acyl-Carrier-Protein S-Acetyltransferase - Acyl-Carrier-Protein S-Malonyltransferase - 3-Oxoacyl-Acyl-Carrier-Protein Synthase - 3-Oxoacyl-Acyl-Carrier-Protein Reductase - 3-Hydroxyacyl-Acyl-Carrier-Protein Dehydratase - Enoyl-Acyl-Carrier-Protein Reductase - Acyl-Acyl-Carrier-Protein Hydrolase ELISA kitFasn ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
