| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
1.88pg/mL |
| Detection Limit: |
3.13~200pg/mL |
| Short Description: |
This rat DCN kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Dcn |
| Gene ID: |
29139 |
| Uniprot ID: |
PGS2_RAT |
| Specificity: |
This kit recognizes Rat DCN in samples. No significant cross-reactivity or interference between Rat DCN and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin. |
| Function | May affect the rate of fibrils formation. May be implicated in the dilatation of the rat cervix. |
| Protein Name | DecorinBone Proteoglycan IiDermatan Sulfate Proteoglycan-IiDspgPg-S2Pg40 |
| Database Links | Reactome: R-RNO-1474228Reactome: -RNO-1971475Reactome: -RNO-2022870Reactome: -RNO-2022923Reactome: -RNO-2024101Reactome: -RNO-3000178 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative CLIA Names | Decorin CLIA kitBone Proteoglycan Ii CLIA kitDermatan Sulfate Proteoglycan-Ii CLIA kitDspg CLIA kitPg-S2 CLIA kitPg40 CLIA kitDcn CLIA kit |
| Specificity | This kit recognizes Rat DCN in samples. No significant cross-reactivity or interference between Rat DCN and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 98-116 | 106 |
| EDTA plasma(n=8) | 97-109 | 104 |
| Cell culture media(n=8) | 92-104 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 11.00 | 24.51 | 79.72 | 10.13 | 25.55 | 78.47 |
| Standard deviation | 0.95 | 1.91 | 9.11 | 1.31 | 2.52 | 7.91 |
| CV (%) | 8.64 | 7.79 | 11.43 | 12.93 | 9.86 | 10.08 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
