| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.38ng/mL |
| Detection Limit: |
0.63~40ng/mL |
| Short Description: |
This rat CYP2E1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Cyp2e1 |
| Gene ID: |
25086 |
| Uniprot ID: |
CP2E1_RAT |
| Specificity: |
This kit recognizes Rat CYP2E1 in samples. No significant cross-reactivity or interference between Rat CYP2E1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics. |
| Protein Name | Cytochrome P450 2e14-Nitrophenol 2-HydroxylaseCypiie1Cytochrome P450-JCytochrome P450rlm6 |
| Database Links | Reactome: R-RNO-211981Reactome: -RNO-211999Reactome: -RNO-9027307Reactome: -RNO-9749641Reactome: -RNO-9753281 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinMicrosome MembraneMitochondrion Inner MembranePost-Translationally Targeted To MitochondriaTomm70 Is Required For The Translocation Across The Mitochondrial Outer MembraneAfter Translocation Into The MatrixAssociates With The Inner Membrane As A Membrane Extrinsic Protein |
| Alternative CLIA Names | Cytochrome P450 2e1 CLIA kit4-Nitrophenol 2-Hydroxylase CLIA kitCypiie1 CLIA kitCytochrome P450-J CLIA kitCytochrome P450rlm6 CLIA kitCyp2e1 CLIA kitCyp2e CLIA kitCyp2e-1 CLIA kit |
| Specificity | This kit recognizes Rat CYP2E1 in samples. No significant cross-reactivity or interference between Rat CYP2E1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-100 | 93 |
| EDTA plasma(n=8) | 100-116 | 106 |
| Cell culture media(n=8) | 102-114 | 108 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.15 | 4.45 | 14.26 | 2.02 | 4.89 | 14.88 |
| Standard deviation | 0.26 | 0.36 | 1.17 | 0.18 | 0.37 | 1.28 |
| CV (%) | 12.09 | 8.09 | 8.20 | 8.91 | 7.57 | 8.60 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
