| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This rat CX3CL1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Cx3cl1 |
| Gene ID: |
89808 |
| Uniprot ID: |
X3CL1_RAT |
| Specificity: |
This kit recognizes Rat CX3CL1 in samples. No significant cross-reactivity or interference between Rat CX3CL1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Highest levels in brain (neurons). Significant levels in kidney, heart, lung and adrenal gland. |
| Post Translational Modifications | A soluble short form may be released by proteolytic cleavage from the long membrane-anchored form. |
| Function | Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1. The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis. Regulates leukocyte adhesion and migration processes at the endothelium. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1. Processed fractalkine: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. Fractalkine: The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. |
| Protein Name | FractalkineC-X3-C Motif Chemokine 1Cx3c Membrane-Anchored ChemokineNeurotactinSmall-Inducible Cytokine D1 Cleaved Into - Processed Fractalkine |
| Database Links | Reactome: R-RNO-380108Reactome: -RNO-418594 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinProcessed Fractalkine: Secreted |
| Alternative CLIA Names | Fractalkine CLIA kitC-X3-C Motif Chemokine 1 CLIA kitCx3c Membrane-Anchored Chemokine CLIA kitNeurotactin CLIA kitSmall-Inducible Cytokine D1 Cleaved Into - Processed Fractalkine CLIA kitCx3cl1 CLIA kitAcc1 CLIA kitFkn CLIA kitScyd1 CLIA kit |
| Specificity | This kit recognizes Rat CX3CL1 in samples. No significant cross-reactivity or interference between Rat CX3CL1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-104 | 97 |
| EDTA plasma(n=8) | 90-106 | 97 |
| Cell culture media(n=8) | 87-97 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 106.10 | 261.11 | 698.69 | 98.92 | 250.55 | 757.52 |
| Standard deviation | 10.13 | 30.24 | 69.87 | 9.60 | 21.22 | 59.84 |
| CV (%) | 9.55 | 11.58 | 10.00 | 9.70 | 8.47 | 7.90 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
