| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
75pg/mL |
| Detection Limit: |
125~8000pg/mL |
| Short Description: |
This rat CTSL kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Ctsl |
| Gene ID: |
25697 |
| Uniprot ID: |
CATL1_RAT |
| Specificity: |
This kit recognizes Rat CTSL in samples. No significant cross-reactivity or interference between Rat CTSL and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Both mature cathepsin L1 and procathepsin L are found in the upper epidermis. The lower epidermis predominantly contains procathepsin L. In seminiferous tubules expression is greater at stages VI-VII than at stages IX-XII. |
| Post Translational Modifications | During export along the endocytic pathway, pro-CTSL undergoes several proteolytic cleavages to generate the CTSL single-chain and two-chain mature forms, composed of a heavy chain linked to a light chain by disulfide bonds. Autocleavage.produces the single-chain CTSL after cleavage of the propeptide. The cleavage can be intermolecular. |
| Function | Thiol protease important for the overall degradation of proteins in lysosomes. Plays a critical for normal cellular functions such as general protein turnover, antigen processing and bone remodeling. Involved in the solubilization of cross-linked TG/thyroglobulin and in the subsequent release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen. In neuroendocrine chromaffin cells secretory vesicles, catalyzes the prohormone proenkephalin processing to the active enkephalin peptide neurotransmitter. In thymus, regulates CD4(+) T cell positive selection by generating the major histocompatibility complex class II (MHCII) bound peptide ligands presented by cortical thymic epithelial cells. Also mediates invariant chain processing in cortical thymic epithelial cells. Major elastin-degrading enzyme at neutral pH. Accumulates as a mature and active enzyme in the extracellular space of antigen presenting cells (APCs) to regulate degradation of the extracellular matrix in the course of inflammation. Secreted form generates endostatin from COL18A1. Critical for cardiac morphology and function. Plays an important role in hair follicle morphogenesis and cycling, as well as epidermal differentiation. Required for maximal stimulation of steroidogenesis by TIMP1. |
| Protein Name | Procathepsin LCathepsin L1Cyclic Protein 2Cp-2Major Excreted ProteinMep Cleaved Into - Cathepsin L - Cathepsin L Heavy Chain - Cathepsin L Light Chain |
| Database Links | Reactome: R-RNO-1442490Reactome: -RNO-1679131Reactome: -RNO-2022090Reactome: -RNO-2132295Reactome: -RNO-8939242 |
| Cellular Localisation | LysosomeApical Cell MembranePeripheral Membrane ProteinExtracellular SideCytoplasmic VesicleSecretory VesicleChromaffin GranuleSecretedExtracellular SpaceLocalizes To The Apical Membrane Of Thyroid Epithelial CellsReleased At Extracellular Space By Activated Dendritic Cells And Macrophages |
| Alternative CLIA Names | Procathepsin L CLIA kitCathepsin L1 CLIA kitCyclic Protein 2 CLIA kitCp-2 CLIA kitMajor Excreted Protein CLIA kitMep Cleaved Into - Cathepsin L - Cathepsin L Heavy Chain - Cathepsin L Light Chain CLIA kitCtsl CLIA kitCtsl1 CLIA kit |
| Specificity | This kit recognizes Rat CTSL in samples. No significant cross-reactivity or interference between Rat CTSL and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 102-116 | 108 |
| EDTA plasma(n=8) | 88-103 | 94 |
| Cell culture media(n=8) | 99-115 | 106 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 378.31 | 974.94 | 2718.88 | 340.48 | 893.10 | 2887.50 |
| Standard deviation | 42.37 | 84.14 | 309.14 | 33.61 | 84.84 | 310.98 |
| CV (%) | 11.20 | 8.63 | 11.37 | 9.87 | 9.50 | 10.77 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
