| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.065ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This CRN Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
Corin |
| Gene ID: |
289596 |
| Uniprot ID: |
CORIN_RAT |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | Specifically expressed in heart. Also detected in kidney, aorta, brain and testis. In kidney, present in epithelial cells, with segmental expression in the proximal tubule, thick ascending limb, connecting tubule, and throughout the collecting duct (at protein level). |
| Post Translational Modifications | N-glycosylated.required for processing and activation. Activated through proteolytic processing by a trypsin-like protease.cleaved into a N-terminal propeptide and an activated corin protease fragment. Atrial natriuretic peptide-converting enzyme, 180 kDa soluble fragment is produced by cleavage by ADAM10. Cleavage by ADAM10 to produce soluble 180 kDa soluble fragment takes place after the transmembrane region and before FZ 1 (Probable). A disulfide bond links the activated corin protease fragment and the N-terminal propeptide. The disulfide bond also links the activated corin protease fragment with Atrial natriuretic peptide-converting enzyme, 180 kDa soluble fragment (Probable). |
| Function | Serine-type endopeptidase involved in atrial natriuretic peptide (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide. |
| Protein Name | Atrial Natriuretic Peptide-Converting EnzymeCorinPro-Anp-Converting Enzyme Cleaved Into - Atrial Natriuretic Peptide-Converting Enzyme - N-Terminal Propeptide - Atrial Natriuretic Peptide-Converting Enzyme - Activated Protease Fragment - Atrial Natriuretic Peptide-Converting Enzyme - 180 Kda Soluble Fragment |
| Database Links | Reactome: R-RNO-5578768 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinCytoplasmic VesicleAtrial Natriuretic Peptide-Converting Enzyme180 Kda Soluble Fragment: SecretedSoluble Form Produced Following Cleavage By Adam10 |
| Alternative ELISA Names | Atrial Natriuretic Peptide-Converting Enzyme ELISA kitCorin ELISA kitPro-Anp-Converting Enzyme Cleaved Into - Atrial Natriuretic Peptide-Converting Enzyme - N-Terminal Propeptide - Atrial Natriuretic Peptide-Converting Enzyme - Activated Protease Fragment - Atrial Natriuretic Peptide-Converting Enzyme - 180 Kda Soluble Fragment ELISA kitCorin ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
