| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
56.25ng/mL |
| Detection Limit: |
93.75~6000ng/mL |
| Short Description: |
This rat AVPR1A kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Avpr1a |
| Gene ID: |
25107 |
| Uniprot ID: |
V1AR_RAT |
| Specificity: |
This kit recognizes Rat AVPR1A in samples. No significant cross-reactivity or interference between Rat AVPR1A and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Localized within gonadotropes of the anterior pituitary of the brain. Broadly distributed throughout the cerebral cortex. |
| Post Translational Modifications | Palmitoylated on three cysteine residues, of which only two are identified. |
| Function | Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl-inositol-calcium second messenger system. Involved in social memory formation. |
| Protein Name | Vasopressin V1a ReceptorV1arAvpr V1aAntidiuretic Hormone Receptor 1aVascular/Hepatic-Type Arginine Vasopressin Receptor |
| Database Links | Reactome: R-RNO-388479Reactome: -RNO-416476 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCytoplasmic Vesicle MembraneLocated In Cytoplasmic Vesicles Dispersed Throughout The Cell Cytoplasm And To The Plasma Membrane |
| Alternative CLIA Names | Vasopressin V1a Receptor CLIA kitV1ar CLIA kitAvpr V1a CLIA kitAntidiuretic Hormone Receptor 1a CLIA kitVascular/Hepatic-Type Arginine Vasopressin Receptor CLIA kitAvpr1a CLIA kit |
| Specificity | This kit recognizes Rat Av pr1a in samples. No significant cross-reactivity or interference between Rat Av pr1a and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-99 | 94 |
| EDTA plasma(n=8) | 99-111 | 105 |
| Cell culture media(n=8) | 84-95 | 90 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 333.06 | 672.13 | 2147.47 | 361.89 | 643.00 | 2277.62 |
| Standard deviation | 33.77 | 54.71 | 205.73 | 41.00 | 48.03 | 166.95 |
| CV (%) | 10.14 | 8.14 | 9.58 | 11.33 | 7.47 | 7.33 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
