• Rat ARC (Activity Regulated Cytoskeleton Associated Protein) Sandwich ELISA Kit (STJE0007297)

Rat ARC (Activity Regulated Cytoskeleton Associated Protein) Sandwich ELISA Kit (STJE0007297)

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STJE0007297

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Applications: ELISA
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.051ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This ARC Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: Arc
Gene ID: 54323
Uniprot ID: ARC_RAT
Sample Type: tissue homogenates or other biological fluids.
Tissue Specificity Expressed exclusively in certain parts of the brain including cortex and molecular layer of the hippocampus. Typically expressed at high level in a minority of neurons. Basal expression higher in cortex than in hippocampus, highest in visual cortex.
Post Translational Modifications Palmitoylation anchors the protein into the membrane by allowing direct insertion into the hydrophobic core of the lipid bilayer. Ubiquitinated by UBE3A, leading to its degradation by the proteasome, thereby promoting AMPA receptors (AMPARs) expression at synapses. Ubiquitinated by RNF216 at Lys-268 and Lys-269 limiting ARC protein levels induced by synaptic activity and thus regulating ARC-dependent forms of synaptic plasticity. Phosphorylation at Ser-260 by CaMK2 prevents homooligomerization into virion-like capsids by disrupting an interaction surface essential for high-order oligomerization. Phosphorylation by CaMK2 inhibits synaptic activity.
Function Master regulator of synaptic plasticity that self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer in the nervous system. ARC protein is released from neurons in extracellular vesicles that mediate the transfer of ARC mRNA into new target cells, where ARC mRNA can undergo activity-dependent translation. ARC capsids are endocytosed and are able to transfer ARC mRNA into the cytoplasm of neurons. Acts as a key regulator of synaptic plasticity: required for protein synthesis-dependent forms of long-term potentiation (LTP) and depression (LTD) and for the formation of long-term memory. Regulates synaptic plasticity by promoting endocytosis of AMPA receptors (AMPARs) in response to synaptic activity: this endocytic pathway maintains levels of surface AMPARs in response to chronic changes in neuronal activity through synaptic scaling, thereby contributing to neuronal homeostasis. Acts as a postsynaptic mediator of activity-dependent synapse elimination in the developing cerebellum by mediating elimination of surplus climbing fiber synapses. Accumulates at weaker synapses, probably to prevent their undesired enhancement. This suggests that ARC-containing virion-like capsids may be required to eliminate synaptic material. Required to transduce experience into long-lasting changes in visual cortex plasticity and for long-term memory. Involved in postsynaptic trafficking and processing of amyloid-beta A4 (APP) via interaction with PSEN1. In addition to its role in synapses, also involved in the regulation of the immune system: specifically expressed in skin-migratory dendritic cells and regulates fast dendritic cell migration, thereby regulating T-cell activation.
Protein Name Activity-Regulated Cytoskeleton-Associated Protein
Activity-Regulated Gene 3.1 Protein
Arc/Arg3.1
Arg3.1
Database Links
Cellular Localisation Extracellular Vesicle Membrane
Lipid-Anchor
Postsynaptic Cell Membrane
Synapse
Postsynaptic Density
Early Endosome Membrane
Cell Projection
Dendrite
Cytoplasm
Cytoskeleton
Cell Cortex
Dendritic Spine
Cytoplasmic Vesicle
Secretory Vesicle
Acrosome
Clathrin-Coated Vesicle Membrane
Forms Virion-Like Extracellular Vesicles That Are Released From Neurons
Enriched In Postsynaptic Density Of Dendritic Spines
Targeted To Inactive Synapses Following Interaction With Camk2b In The Kinase Inactive State
Accumulation At Weaker Synapses May Be Required To Prevent Their Undesired Enhancement
Associated With The Cell Cortex Of Neuronal Soma And Dendrites
Associated With The Sperm Tail
Alternative ELISA Names Activity-Regulated Cytoskeleton-Associated Protein ELISA kit
Activity-Regulated Gene 3.1 Protein ELISA kit
Arc/Arg3.1 ELISA kit
Arg3.1 ELISA kit
Arc ELISA kit
output

Information sourced from Uniprot.org

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