Western Blot Antibodies | St John's Laboratory

Western blot analysis of extracts of various cell lines, using CDC25C antibody (STJ23026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-CDC25C antibody (20-130) (STJ23026)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

Choose Options

Western blot analysis using CDC25C monoclonal antibody against HeLa (1) , K562 (2) , PC-3 (3) , HEK293 (4) and Raw264.7 (5) cell lysate.

Anti-CDC25C antibody [1F12] (STJ97944)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

€180.00 - €435.00

Choose Options

Western blot analysis of various cells using Cdc27 Polyclonal Antibody

Anti-CDC27 antibody (361-410 aa) (STJ92167)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

Choose Options

Immunohistochemistry analysis of paraffin-embedded mouse heart using CDC27 antibody (STJ23027) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CDC27 antibody (551-830) (STJ23027)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

Choose Options

Western blot analysis of extracts of various cell lines, using CDC27 Rabbit monoclonal antibody (STJ11101851) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CDC27 antibody (702-824) [S1MR] (STJ11101851)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€161.88 - €718.13

Choose Options

Western blot analysis using CDC27 monoclonal antibody against CDC27-hIgGFc transfected HEK293 cell.

Anti-CDC27 antibody [5C12] (STJ97945)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

€180.00 - €435.00

Choose Options

Western blot analysis of K562, PC-3, mouse liver cells using Cdc34 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-CDC34 antibody (121-170 aa) (STJ96554)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

Choose Options

Immunofluorescence analysis of BALB-3T3 cells using CDC34 Rabbit polyclonal antibody (STJ27410) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CDC34 antibody (150-236) (STJ27410)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

Choose Options

Western blot analysis of various lysates using CDC34 Rabbit mAb (STJ11102546) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-CDC34 antibody (20-236) [S6MR] (STJ11102546)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

Choose Options

Western blot analysis of extracts of various cell lines, using CDC37 Rabbit monoclonal antibody (STJ11102109) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CDC37 antibody (1-100) [S9MR] (STJ11102109)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

Choose Options

Western blot analysis of various lysates using CDC37 Rabbit polyclonal antibody (STJ23029) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CDC37 antibody (1-160) (STJ23029)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

Choose Options

Western blot analysis of various cells using Cdc37 Polyclonal Antibody

Anti-CDC37 antibody (1-50 aa) (STJ92170)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

Choose Options

Western blot analysis of various lysates using CDC37L1 Rabbit pAb (STJ116262) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-CDC37L1 antibody (2-240) (STJ116262)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

Choose Options

Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CDC37L1 antibody (80-160 aa) (STJ92171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€60.00 - €315.00

Choose Options

Anti-CDC40 antibody (1-100 aa) (STJ11105981)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

Choose Options

Immunohistochemistry analysis of CDC40 in paraffin-embedded mouse colon tissue using CDC40 Rabbit monoclonal antibody (STJ11103139) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-CDC40 antibody (1-100) [S9MR] (STJ11103139)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

Choose Options

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-CDC40 antibody (179-228 aa) (STJ92172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

Choose Options

Immunofluorescence analysis of NIH-3T3 cells using CDC42 Rabbit polyclonal antibody (STJ29840) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-CDC42 antibody (118-191) (STJ29840)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

Choose Options

Western blot analysis of extracts of various cell lines, using CDC42 antibody (STJ11101566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CDC42 antibody (60-191) [S6MR] (STJ11101566)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

Choose Options

Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CDC42 antibody (80-160 aa) (STJ191678)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

Choose Options

Anti-CDC42 antibody [M152] (STJA0005338)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

€399.38

Choose Options

Anti-CDC42 antibody [M430] (STJA0005339)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

€399.38

Choose Options

Anti-CDC42BPA antibody (300-400) (STJ11100349)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

Choose Options

Western blot analysis of various cells using MRCK Alpha Polyclonal Antibody

Anti-CDC42BPA antibody (580-660) (STJ94192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

Choose Options

Western blot analysis of various cells using MRCK Beta Polyclonal Antibody

Anti-CDC42BPB antibody (1641-1690) (STJ94193)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

Choose Options

Western Blot analysis of HEK293 lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-CDC42BPG antibody (1370-1450 aa) (STJ191748)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

Choose Options

Immunohistochemistry analysis of paraffin-embedded mouse heart using CDC42EP1 Rabbit monoclonal antibody (STJ11103098) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-CDC42EP1 antibody (292-391) [S8MR] (STJ11103098)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

Choose Options

Anti-CDC42EP1 antibody (STJ190675)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

Choose Options

Western blot analysis of K562 cells using Cdc42EP2 Polyclonal Antibody

Anti-CDC42EP2 antibody (10-59 aa) (STJ92173)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

Choose Options

Western blot analysis of various lysates using CDC42EP3 Rabbit pAb (STJ110778) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-CDC42EP3 antibody (1-254) (STJ110778)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

Choose Options

Western blot analysis of K562 cells using Cdc42EP3 Polyclonal Antibody

Anti-CDC42EP3 antibody (N-Term) (STJ92174)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

Choose Options

Western blot analysis of lysates from Jurkat cells, using BORG4 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-CDC42EP4 antibody (201-250 aa) (STJ92175)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

Choose Options

Western blot analysis of various cells using Cdc42EP5 Polyclonal Antibody diluted at 1：500

Anti-CDC42EP5 antibody (1-50 aa) (STJ92176)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

Choose Options

Western blot analysis of extracts of various cell lines, using cdc45 antibody (STJ26198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-CDC45 antibody (1-270) (STJ26198)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

Choose Options

Immunofluorescence analysis of A-549 cells using cdc45 antibody (STJ118472). Blue: DAPI for nuclear staining.

Anti-cdc45 antibody (1-566) (STJ118472)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

€140.63 - €655.63

Choose Options

Anti-CDC45 antibody (90-170 aa) (STJ190408)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

Choose Options

STJ73340 (0. 3µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CDC48/VCP/YDL126C-Baker NA s Yeast antibody (128-142) (STJ73340)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

S.cerevisiae S288c/Human/Mouse/Rat/Dog/Cow

€390.63

Choose Options

STJ73341 (0. 01µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CDC48/YDL126C antibody (Internal) (STJ73341)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

S.cerevisiae S288c/Human/Mouse/Rat/Dog/Cow

€390.63

Choose Options

Western blot analysis of extracts of various cell lines, using CDC5L antibody (STJ27506) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CDC5L antibody (563-802) (STJ27506)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

Choose Options

Western blot analysis of lysates from HeLa cells, using Cdc6 Rabbit polyclonal antibody (STJ11100206) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CDC6 antibody (1-560) (STJ11100206)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

Choose Options

Western blot analysis of lysates from K562 cells, using CDC6 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-CDC6 antibody (491-540 aa) (STJ92181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

Choose Options

STJ72204 (0. 01µg/ml) staining of nuclear Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CDC6 antibody (Internal) (STJ72204)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

€390.63

Choose Options

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-CDC7 antibody (1-50 aa) (STJ92183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

Choose Options

Western blot analysis of extracts of various cell lines, using CDC7 antibody (STJ28305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CDC7 antibody (275-574) (STJ28305)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

Choose Options

Western blot analysis of various lysates, using HRPT2/ CDC73 Rabbit monoclonal antibody (STJ11105154) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CDC73 antibody (300-400) [S5154RM] (STJ11105154)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

Choose Options

Western blot analysis of lysates from mouse testis, using HRPT2/ CDC73 Rabbit polyclonal antibody (STJ11103808). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CDC73 antibody (435-505) (STJ11103808)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

Choose Options

Western blot analysis of 1, mouse-liver 2, mouse-brain cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-CDC73 antibody (51-100 aa) (STJ98854)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

Choose Options

Western blot analysis of various cells using Repo-Man Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-CDCA2 antibody (511-560 aa) (STJ95418)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

€60.00 - €315.00

Choose Options