• Mouse Vegfc (Vascular endothelial growth factor C) Sandwich ELISA (STJE0005095)

Mouse Vegfc (Vascular endothelial growth factor C) Sandwich ELISA (STJE0005095)

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STJE0005095

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This Vegfc Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Vegfc
Gene ID: 22341
Uniprot ID: VEGFC_MOUSE
Specificity: This kit recognizes Mouse VEGF-C in samples. No significant cross-reactivity or interference between Mouse VEGF-C and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3, but only the fully processed form could activate VEGFR-2. VEGF-C first form an antiparallel homodimer linked by disulfide bonds. Before secretion, a cleavage occurs between Arg-223 and Ser-224 producing a heterotetramer. The next extracellular step of the processing removes the N-terminal propeptide. Finally the mature VEGF-C is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
Function Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates KDR/VEGFR2 and FLT4/VEGFR3 receptors.
Protein Name Vascular Endothelial Growth Factor C
Vegf-C
Flt4 Ligand
Flt4-L
Vascular Endothelial Growth Factor-Related Protein
Vrp
Database Links Reactome: R-MMU-114608
Reactome: -MMU-194313
Reactome: -MMU-195399
Cellular Localisation Secreted
Alternative ELISA Names Vascular Endothelial Growth Factor C ELISA kit
Vegf-C ELISA kit
Flt4 Ligand ELISA kit
Flt4-L ELISA kit
Vascular Endothelial Growth Factor-Related Protein ELISA kit
Vrp ELISA kit
Vegfc ELISA kit
Specificity This kit recognizes Mouse VEGF-C in samples. No significant cross-reactivity or interference between Mouse VEGF-C and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-106 99
EDTA plasma (n=8) 91-105 99
Cell culture media (n=8) 86-98 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 99.00 219.80 759.20 105.60 204.40 747.80
Standard deviation 5.50 11.90 40.20 5.70 8.80 35.90
CV (%) 5.56 5.41 5.30 5.40 4.31 4.80
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance