Mouse VEGF-A (Vascular Endothelial Cell Growth Factor A) Sandwich ELISA (STJE0019719)

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STJE0019719

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 3.21pg/mL
Detection Limit: 7.81-500pg/mL
Short Description: This VEGF-A Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Vegfa
Gene ID: 22339
Uniprot ID: VEGFA_MOUSE
Specificity: This kit recognizes Mouse VEGF-A in samples. No significant cross-reactivity or interference between Mouse VEGF-A and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Vascular endothelial growth factor A, long form: Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form.
Function N-VEGF: Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death. VEGFA: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Also binds the DEAR/FBXW7-AS1 receptor. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.
Protein Name Vascular Endothelial Growth Factor A - Long Form
L-Vegf
Vascular Permeability Factor
Vpf Cleaved Into - N-Vegf - Vegfa
Database Links Reactome: R-MMU-114608
Reactome: -MMU-194313
Reactome: -MMU-195399
Reactome: -MMU-4420097
Reactome: -MMU-5218921
Cellular Localisation N-Vegf: Cytoplasm
Nucleus
Cytoplasmic In Normoxic Conditions And Localizes To The Nucleus Under Hypoxic Conditions
Vegfa: Secreted
Isoform L-Vegf-2: Endoplasmic Reticulum
Golgi Apparatus
Secreted
Extracellular Space
Extracellular Matrix
Isoform Vegf-1: Secreted
Isoform Vegf-2: Secreted
Isoform Vegf-3: Cell Membrane
Peripheral Membrane Protein
Remains Cell-Surface Associated Unless Released By Heparin
Alternative ELISA Names Vascular Endothelial Growth Factor A - Long Form ELISA kit
L-Vegf ELISA kit
Vascular Permeability Factor ELISA kit
Vpf Cleaved Into - N-Vegf - Vegfa ELISA kit
Vegfa ELISA kit
Vegf ELISA kit
Specificity This kit recognizes Mouse VEGF-A in samples. No significant cross-reactivity or interference between Mouse VEGF-A and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (50×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (50×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 99-105 102
EDTA plasma (n=8) 93-100 96
Cell culture media (n=8) 87-98 90

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 22.41 58.49 215.21 20.44 50.95 220.39
Standard deviation 1.32 2.81 10.16 1.03 2.39 10.01
CV (%) 5.91 4.81 4.72 5.06 4.69 4.54
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance