| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.114ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This TUBa4A Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Tuba4a |
| Gene ID: |
22145 |
| Uniprot ID: |
TBA4A_MOUSE |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Some glutamate residues at the C-terminus are polyglycylated, resulting in polyglycine chains on the gamma-carboxyl group. Glycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering polyglycylation levels increases polyglutamylation, and reciprocally. Cilia and flagella glycylation is required for their stability and maintenance. Flagella glycylation controls sperm motility. Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Glutamylation is also involved in cilia motility. Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly. Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability. Although this tubulin does not encode a C-terminal tyrosine, a C-terminal tyrosine can be added post-translationally by the tubulin tyrosine ligase (TTL). It can then undergo a detyrosination cycle by the tubulin tyrosine carboxypeptidase (KIAA0895L/MATCAP). |
| Function | Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. |
| Protein Name | Tubulin Alpha-4a ChainAlpha-Tubulin 4Alpha-Tubulin Isotype M-Alpha-4Tubulin Alpha-4 Chain |
| Database Links | Reactome: R-MMU-114608Reactome: -MMU-190840Reactome: -MMU-2132295Reactome: -MMU-2467813Reactome: -MMU-2500257Reactome: -MMU-2565942Reactome: -MMU-3371497Reactome: -MMU-380259Reactome: -MMU-380270Reactome: -MMU-380284Reactome: -MMU-380320Reactome: -MMU-437239Reactome: -MMU-5610787Reactome: -MMU-5617833Reactome: -MMU-5620912Reactome: -MMU-5620924Reactome: -MMU-5626467Reactome: -MMU-5663220Reactome: -MMU-6807878Reactome: -MMU-6811434Reactome: -MMU-6811436Reactome: -MMU-68877Reactome: -MMU-8852276Reactome: -MMU-8854518Reactome: -MMU-8955332Reactome: -MMU-9646399Reactome: -MMU-9648025Reactome: -MMU-9668328Reactome: -MMU-983189 |
| Cellular Localisation | CytoplasmCytoskeleton |
| Alternative ELISA Names | Tubulin Alpha-4a Chain ELISA kitAlpha-Tubulin 4 ELISA kitAlpha-Tubulin Isotype M-Alpha-4 ELISA kitTubulin Alpha-4 Chain ELISA kitTuba4a ELISA kitTuba4 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
