• Mouse tPA (Tissue-type Plasminogen Activator) CLIA Kit (STJC0001396)

Mouse tPA (Tissue-type Plasminogen Activator) CLIA Kit (STJC0001396)

SKU:
STJC0001396

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Applications: CLIA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This mouse tPA kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Plat
Gene ID: 18791
Uniprot ID: TPA_MOUSE
Specificity: This kit recognizes Mouse tPA in samples. No significant cross-reactivity or interference between Mouse tPA and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-308 catalyzed by plasmin, tissue kallikrein or factor Xa.
Function Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy.
Protein Name Tissue-Type Plasminogen Activator
T-Pa
T-Plasminogen Activator
Tpa Cleaved Into - Tissue-Type Plasminogen Activator Chain A - Tissue-Type Plasminogen Activator Chain B
Database Links Reactome: R-MMU-186797
Reactome: -MMU-75205
Cellular Localisation Secreted
Extracellular Space
Alternative CLIA Names Tissue-Type Plasminogen Activator CLIA kit
T-Pa CLIA kit
T-Plasminogen Activator CLIA kit
Tpa Cleaved Into - Tissue-Type Plasminogen Activator Chain A - Tissue-Type Plasminogen Activator Chain B CLIA kit
Plat CLIA kit
Specificity This kit recognizes Mouse tPA in samples. No significant cross-reactivity or interference between Mouse tPA and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-103 97
EDTA plasma(n=8) 97-115 105
Cell culture media(n=8) 99-111 105

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 47.76 132.56 408.21 52.31 125.40 372.36
Standard deviation 5.04 9.74 43.56 4.73 12.29 29.23
CV (%) 10.55 7.35 10.67 9.04 9.80 7.85
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance