| Host: |
HEK293 cells |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Mouse TNF-alpha/TNF-NO-tag protein was developed from hek293 cells and has a target region of NO-tag. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: |
Recombinant Mouse TNF-alpha induces cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 29-116 pg/mL, corresponding to a specific activity of 8.62×106-3.45×1 |
| Gene Symbol: |
Tnf |
| Gene ID: |
21926 |
| Uniprot ID: |
TNFA_MOUSE |
| Immunogen Region: |
Leu80-Leu235 |
| Immunogen: |
Recombinant Mouse TNF-alpha/TNF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu80-Leu235) of mouse TNF-alpha/TNF (Accession #NP_038721.1.) fused with no additional amino acid. |
| Function | Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6. Promotes osteoclastogenesis and therefore mediates bone resorption. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. |
| Protein Name | Tumor Necrosis FactorCachectinTnf-AlphaTumor Necrosis Factor Ligand Superfamily Member 2Tnf-A Cleaved Into - Tumor Necrosis Factor - Membrane FormN-Terminal FragmentNtf - Intracellular Domain 1Icd1 - Intracellular Domain 2Icd2 - C-Domain 1 - C-Domain 2 - Tumor Necrosis Factor - Soluble Form |
| Database Links | Reactome: R-MMU-5357786Reactome: -MMU-5357905Reactome: -MMU-5357956Reactome: -MMU-5626978Reactome: -MMU-5668541Reactome: -MMU-75893 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinTumor Necrosis FactorMembrane Form: MembraneSoluble Form: SecretedC-Domain 1: SecretedC-Domain 2: Secreted |
| Alternative Protein Names | Tumor Necrosis Factor proteinCachectin proteinTnf-Alpha proteinTumor Necrosis Factor Ligand Superfamily Member 2 proteinTnf-A Cleaved Into - Tumor Necrosis Factor - Membrane Form proteinN-Terminal Fragment proteinNtf - Intracellular Domain 1 proteinIcd1 - Intracellular Domain 2 proteinIcd2 - C-Domain 1 - C-Domain 2 - Tumor Necrosis Factor - Soluble Form proteinTnf proteinTnfa proteinTnfsf2 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
