| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
6.8pg/mL |
| Detection Limit: |
15.6-1000pg/mL |
| Short Description: |
This SCF Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
Kitlg |
| Gene ID: |
17311 |
| Uniprot ID: |
SCF_MOUSE |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | Expressed in the cochlea. |
| Post Translational Modifications | A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain. |
| Function | Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. |
| Protein Name | Kit LigandHematopoietic Growth Factor KlMast Cell Growth FactorMgfSteel FactorStem Cell FactorScfC-Kit Ligand Cleaved Into - Soluble Kit LigandSkitlg |
| Database Links | Reactome: R-MMU-1257604Reactome: -MMU-1433557Reactome: -MMU-1433559Reactome: -MMU-5673001Reactome: -MMU-6811558 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 2: CytoplasmCytoplasmCytoskeletonCell MembraneCell ProjectionLamellipodiumFilopodiumSoluble Kit Ligand: Secreted |
| Alternative ELISA Names | Kit Ligand ELISA kitHematopoietic Growth Factor Kl ELISA kitMast Cell Growth Factor ELISA kitMgf ELISA kitSteel Factor ELISA kitStem Cell Factor ELISA kitScf ELISA kitC-Kit Ligand Cleaved Into - Soluble Kit Ligand ELISA kitSkitlg ELISA kitKitlg ELISA kitKitl ELISA kitMgf ELISA kitSl ELISA kitSlf ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
