• Mouse RXRa (Retinoid X Receptor Alpha) Sandwich ELISA Kit Ready-To-Use (STJE0018345)

Mouse RXRa (Retinoid X Receptor Alpha) Sandwich ELISA Kit Ready-To-Use (STJE0018345)

SKU:
STJE0018345

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Applications: ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.067ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This RXRa Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: Rxra
Gene ID: 20181
Uniprot ID: RXRA_MOUSE
Immunogen Region: Ready-To-Use
Sample Type: tissue homogenates or other biological fluids.
Tissue Specificity Expressed in macrophages (at protein level).
Post Translational Modifications Acetylated by EP300.acetylation enhances DNA binding and transcriptional activity. Phosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constitutively phosphorylated on Ser-22 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-61, Ser-75, Thr-87 and Ser-265. Phosphorylated on Ser-28, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA. Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6.
Function Receptor for retinoic acid that acts as a transcription factor. Forms homo- or heterodimers with retinoic acid receptors (RARs) and binds to target response elements in response to their ligands, all-trans or 9-cis retinoic acid, to regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5 to regulate transcription. The high affinity ligand for retinoid X receptors (RXRs) is 9-cis retinoic acid. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone deacetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and coactivators are recruited leading to transcriptional activation. Serves as a common heterodimeric partner for a number of nuclear receptors, such as RARA, RARB and PPARA. The RXRA/RARB heterodimer can act as a transcriptional repressor or transcriptional activator, depending on the RARE DNA element context. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. Together with RARA, positively regulates microRNA-10a expression, thereby inhibiting the GATA6/VCAM1 signaling response to pulsatile shear stress in vascular endothelial cells. Acts as an enhancer of RARA binding to RARE DNA element. May facilitate the nuclear import of heterodimerization partners such as VDR and NR4A1. Promotes myelin debris phagocytosis and remyelination by macrophages. Plays a role in the attenuation of the innate immune system in response to viral infections, possibly by negatively regulating the transcription of antiviral genes such as type I IFN genes. Involved in the regulation of calcium signaling by repressing ITPR2 gene expression, thereby controlling cellular senescence.
Protein Name Retinoic Acid Receptor Rxr-Alpha
Nuclear Receptor Subfamily 2 Group B Member 1
Retinoid X Receptor Alpha
Database Links Reactome: R-MMU-159418
Reactome: -MMU-192105
Reactome: -MMU-193368
Reactome: -MMU-193807
Reactome: -MMU-200425
Reactome: -MMU-204174
Reactome: -MMU-211976
Reactome: -MMU-381340
Reactome: -MMU-383280
Reactome: -MMU-400206
Reactome: -MMU-4090294
Reactome: -MMU-5362517
Reactome: -MMU-9029569
Reactome: -MMU-9616222
Reactome: -MMU-9623433
Reactome: -MMU-9707564
Cellular Localisation Nucleus
Cytoplasm
Mitochondrion
Localization To The Nucleus Is Enhanced By Vitamin D3
Nuclear Localization May Be Enhanced By The Interaction With Heterodimerization Partner Vdr
Translocation To The Mitochondrion Upon Interaction With Nr4a1
Increased Nuclear Localization Upon Pulsatile Shear Stress
Alternative ELISA Names Retinoic Acid Receptor Rxr-Alpha ELISA kit
Nuclear Receptor Subfamily 2 Group B Member 1 ELISA kit
Retinoid X Receptor Alpha ELISA kit
Rxra ELISA kit
Nr2b1 ELISA kit
output

Information sourced from Uniprot.org

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