• Mouse PKM2 (Pyruvate Kinase, Muscle) Sandwich ELISA Kit Ready-To-Use (STJE0017895)

Mouse PKM2 (Pyruvate Kinase, Muscle) Sandwich ELISA Kit Ready-To-Use (STJE0017895)

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STJE0017895

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Applications: ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.144ng/mL
Detection Limit: 0.312-20ng/mL
Short Description: This PKM2 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: Pkm
Gene ID: 18746
Uniprot ID: KPYM_MOUSE
Immunogen Region: Ready-To-Use
Sample Type: serum, plasma or other biological fluids.
Tissue Specificity Embryonic stem cells and embryonal carcinoma cells.
Post Translational Modifications ISGylated. Under hypoxia, hydroxylated by EGLN3. Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy. Isoform M2: Acetylated at Lys-433 by EP300, leading to impair phosphoenolpyruvate substrate-binding and promote its homodimerization and subsequent translocation to the nucleus. Deacetylation at Lys-433 by SIRT6 promotes its nuclear export into the cytoplasm, leading to suppress its nuclear localization and oncogenic function. FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.
Function Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. Isoform M2: Isoform specifically expressed during embryogenesis that has low pyruvate kinase activity by itself and requires allosteric activation by D-fructose 1,6-bisphosphate (FBP) for pyruvate kinase activity. In addition to its pyruvate kinase activity in the cytoplasm, also acts as a regulator of transcription in the nucleus by acting as a protein kinase. Translocates into the nucleus in response to various signals, such as EGF receptor activation, and homodimerizes, leading to its conversion into a protein threonine- and tyrosine-protein kinase. Catalyzes phosphorylation of STAT3 at 'Tyr-705' and histone H3 at 'Thr-11' (H3T11ph), leading to activate transcription. Its ability to activate transcription plays a role in cancer cells by promoting cell proliferation and promote tumorigenesis. Promotes the expression of the immune checkpoint protein CD274 in BMAL1-deficient macrophages. May also act as a translation regulator for a subset of mRNAs, independently of its pyruvate kinase activity: associates with subpools of endoplasmic reticulum-associated ribosomes, binds directly to the mRNAs translated at the endoplasmic reticulum and promotes translation of these endoplasmic reticulum-destined mRNAs. Plays a role in caspase independent cell death of tumor cells. Isoform M1: Pyruvate kinase isoform expressed in adult tissues, which replaces isoform M2 after birth. In contrast to isoform M2, has high pyruvate kinase activity by itself and does not require allosteric activation by D-fructose 1,6-bisphosphate (FBP) for activity.
Protein Name Pyruvate Kinase Pkm
Pyruvate Kinase Muscle Isozyme
Threonine-Protein Kinase Pkm2
Tyrosine-Protein Kinase Pkm2
Database Links Reactome: R-MMU-6798695
Reactome: -MMU-70171
Cellular Localisation Isoform M2: Cytoplasm
Nucleus
Translocates To The Nucleus In Response To Various Signals
Such As Egf Receptor Activation Or Apoptotic Stimuli
Nuclear Translocation Is Promoted By Acetylation By Ep300
Deacetylation By Sirt6 Promotes Its Nuclear Export In A Process Dependent Of Xpo4
Thereby Suppressing Its Ability To Activate Transcription And Promote Tumorigenesis
Isoform M1: Cytoplasm
Alternative ELISA Names Pyruvate Kinase Pkm ELISA kit
Pyruvate Kinase Muscle Isozyme ELISA kit
Threonine-Protein Kinase Pkm2 ELISA kit
Tyrosine-Protein Kinase Pkm2 ELISA kit
Pkm ELISA kit
Pk3 ELISA kit
Pkm2 ELISA kit
Pykm ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance