| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.042ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This PDHa Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Pdha1 |
| Gene ID: |
18597 |
| Uniprot ID: |
ODPA_MOUSE |
| Sample Type: |
serum, plasma, tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | In all tissues, but in very low amount in testis. |
| Post Translational Modifications | Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme.for this phosphorylation at a single site is sufficient. Phosphorylation at Ser-293 interferes with access to active site, and thereby inactivates the enzyme. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation. Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3. |
| Function | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. |
| Protein Name | Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - MitochondrialPdhe1-A Type I |
| Database Links | Reactome: R-MMU-204174Reactome: -MMU-389661Reactome: -MMU-5362517Reactome: -MMU-70268 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative ELISA Names | Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - Mitochondrial ELISA kitPdhe1-A Type I ELISA kitPdha1 ELISA kitPdha-1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
