| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This Pdgfd Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Pdgfd |
| Gene ID: |
71785 |
| Uniprot ID: |
PDGFD_MOUSE |
| Specificity: |
This kit recognizes Mouse PDGF-D in samples. No significant cross-reactivity or interference between Mouse PDGF-D and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Activated by proteolytic cleavage. Proteolytic removal of the N-terminal CUB domain releasing the core domain is necessary for unmasking the receptor-binding epitopes of the core domain. Cleavage after Arg-247 or Arg-249 by urokinase plasminogen activator gives rise to the active form. |
| Function | Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Has oncogenic potential and can induce tumor formation. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix. |
| Protein Name | Platelet-Derived Growth Factor DPdgf-DSpinal Cord-Derived Growth Factor BScdgf-B Cleaved Into - Platelet-Derived Growth Factor D - Latent FormPdgfd Latent Form - Platelet-Derived Growth Factor D - Receptor-Binding FormPdgfd Receptor-Binding Form |
| Database Links | Reactome: R-MMU-186797 |
| Cellular Localisation | SecretedReleased By Platelets Upon Wounding |
| Alternative ELISA Names | Platelet-Derived Growth Factor D ELISA kitPdgf-D ELISA kitSpinal Cord-Derived Growth Factor B ELISA kitScdgf-B Cleaved Into - Platelet-Derived Growth Factor D - Latent Form ELISA kitPdgfd Latent Form - Platelet-Derived Growth Factor D - Receptor-Binding Form ELISA kitPdgfd Receptor-Binding Form ELISA kitPdgfd ELISA kitScdgfb ELISA kit |
| Specificity | This kit recognizes Mouse PDGF-D in samples. No significant cross-reactivity or interference between Mouse PDGF-D and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-102 | 96 |
| EDTA plasma (n=8) | 83-98 | 90 |
| Cell culture media (n=8) | 91-107 | 99 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 48.33 | 105.90 | 455.94 | 47.94 | 109.30 | 454.69 |
| Standard deviation | 2.66 | 5.36 | 22.93 | 3.21 | 5.32 | 18.28 |
| CV (%) | 5.50 | 5.06 | 5.03 | 6.70 | 4.87 | 4.02 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
