• Mouse PCK1 (Phosphoenolpyruvate Carboxykinase 1, Soluble) Sandwich ELISA Kit Ready-To-Use (STJE0017690)

Mouse PCK1 (Phosphoenolpyruvate Carboxykinase 1, Soluble) Sandwich ELISA Kit Ready-To-Use (STJE0017690)

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STJE0017690

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Applications: ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.058ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This PCK1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: Pck1
Gene ID: 18534
Uniprot ID: PCKGC_MOUSE
Immunogen Region: Ready-To-Use
Sample Type: tissue homogenates or other biological fluids.
Tissue Specificity
Post Translational Modifications Acetylated. Lysine acetylation by p300/EP300 is increased on high glucose conditions and promotes ubiquitination by UBR5, acetylation is enhanced in the presence of BAG6. Deacetylated by SIRT2. Deacetylated by SIRT1. Ubiquitination by UBR5 leads to proteasomal degradation. Phosphorylated in a GSK3B-mediated pathway.phosphorylation affects the efficiency of SIRT1-mediated deacetylation, and regulates PCK1 ubiquitination and degradation. Phosphorylation at Ser-90 by AKT1 reduces the binding affinity to oxaloacetate and promotes the protein kinase activity: phosphorylated PCK1 translocates to the endoplasmic reticulum, where it phosphorylates INSIG1 and INSIG2.
Function Cytosolic phosphoenolpyruvate carboxykinase that catalyzes the reversible decarboxylation and phosphorylation of oxaloacetate (OAA) and acts as the rate-limiting enzyme in gluconeogenesis. Regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle. At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate. Acts as a regulator of formation and maintenance of memory CD8(+) T-cells: up-regulated in these cells, where it generates phosphoenolpyruvate, via gluconeogenesis. The resultant phosphoenolpyruvate flows to glycogen and pentose phosphate pathway, which is essential for memory CD8(+) T-cells homeostasis. In addition to the phosphoenolpyruvate carboxykinase activity, also acts as a protein kinase when phosphorylated at Ser-90: phosphorylation at Ser-90 by AKT1 reduces the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as donor. The protein kinase activity regulates lipogenesis: upon phosphorylation at Ser-90, translocates to the endoplasmic reticulum and catalyzes phosphorylation of INSIG proteins (INSIG1 and INSIG2), thereby disrupting the interaction between INSIG proteins and SCAP and promoting nuclear translocation of SREBP proteins (SREBF1/SREBP1 or SREBF2/SREBP2) and subsequent transcription of downstream lipogenesis-related genes.
Protein Name Phosphoenolpyruvate Carboxykinase - Cytosolic Gtp
Pepck-C
Serine-Protein Kinase Pck1
Database Links Reactome: R-MMU-70263
Cellular Localisation Cytoplasm
Cytosol
Endoplasmic Reticulum
Phosphorylation At Ser-90 Promotes Translocation To The Endoplasmic Reticulum
Alternative ELISA Names Phosphoenolpyruvate Carboxykinase - Cytosolic Gtp ELISA kit
Pepck-C ELISA kit
Serine-Protein Kinase Pck1 ELISA kit
Pck1 ELISA kit
Pepck ELISA kit
output

Information sourced from Uniprot.org

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