• Mouse P1CP (Procollagen 1 C-Terminal Propeptide) Sandwich ELISA (STJE0004360)

Mouse P1CP (Procollagen 1 C-Terminal Propeptide) Sandwich ELISA (STJE0004360)

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STJE0004360

£194.50 - £368.50
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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This P1CP Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Col1a1
Gene ID: 12842
Uniprot ID: CO1A1_MOUSE
Specificity: This kit recognizes Mouse P 1 CP in samples. No significant cross-reactivity or interference between Mouse P 1 CP and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Contains mostly 4-hydroxyproline. Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline. Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains. O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide.
Function Type I collagen is a member of group I collagen (fibrillar forming collagen).
Protein Name Collagen Alpha-1(I Chain
Alpha-1 Type I Collagen
Database Links Reactome: R-MMU-114604
Reactome: -MMU-1442490
Reactome: -MMU-1474244
Reactome: -MMU-1650814
Reactome: -MMU-198933
Reactome: -MMU-2022090
Reactome: -MMU-202733
Reactome: -MMU-216083
Reactome: -MMU-2243919
Reactome: -MMU-3000171
Reactome: -MMU-3000178
Reactome: -MMU-430116
Reactome: -MMU-75892
Reactome: -MMU-76009
Reactome: -MMU-8874081
Reactome: -MMU-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative ELISA Names Collagen Alpha-1(I Chain ELISA kit
Alpha-1 Type I Collagen ELISA kit
Col1a1 ELISA kit
Cola1 ELISA kit
Specificity This kit recognizes Mouse PⅠCP in samples. No significant cross-reactivity or interference between Mouse PⅠCP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-100 94
EDTA plasma (n=8) 94-112 102
Cell culture media (n=8) 93-107 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 103.26 321.15 863.49 94.71 313.21 836.71
Standard deviation 6.69 13.87 41.36 6.00 15.72 38.40
CV (%) 6.48 4.32 4.79 6.34 5.02 4.59
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance