| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.057ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This OCT4 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Pou5f1 |
| Gene ID: |
18999 |
| Uniprot ID: |
PO5F1_MOUSE |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Expressed the totipotent and pluripotent stem cells of the pregastrulation embryo. Also expressed in primordial germ cells and in the female germ line. Absent from adult tissues. |
| Post Translational Modifications | Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression. Ubiquitinated.undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. ERK1/2-mediated phosphorylation at Ser-106 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-228 and Ser-229 decrease DNA-binding and alters ability to activate transcription. JNK1/2-mediated phosphorylation at Ser-347 promotes proteasomal degradation. |
| Function | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. |
| Protein Name | Pou Domain - Class 5 - Transcription Factor 1Nf-A3Octamer-Binding Protein 3Oct-3Octamer-Binding Protein 4Oct-4Octamer-Binding Transcription Factor 3Otf-3 |
| Database Links | |
| Cellular Localisation | CytoplasmNucleusExpressed In A Diffuse And Slightly Punctuate PatternColocalizes With Mapk8 And Mapk9 In The Nucleus |
| Alternative ELISA Names | Pou Domain - Class 5 - Transcription Factor 1 ELISA kitNf-A3 ELISA kitOctamer-Binding Protein 3 ELISA kitOct-3 ELISA kitOctamer-Binding Protein 4 ELISA kitOct-4 ELISA kitOctamer-Binding Transcription Factor 3 ELISA kitOtf-3 ELISA kitPou5f1 ELISA kitOct-3 ELISA kitOct-4 ELISA kitOtf-3 ELISA kitOtf3 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
