• Mouse NEIL3 (Nei Endonuclease VIII Like Protein 3) Sandwich ELISA Kit Ready-To-Use (STJE0017299)

Mouse NEIL3 (Nei Endonuclease VIII Like Protein 3) Sandwich ELISA Kit Ready-To-Use (STJE0017299)

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STJE0017299

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Applications: ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.061ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This NEIL3 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: Neil3
Gene ID: 234258
Uniprot ID: NEIL3_MOUSE
Immunogen Region: Ready-To-Use
Sample Type: tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity Expressed in testis, thymus, spleen and bone marrow. In young mice, expressed at higher levels in thymocytes than splenocytes. At 12 dpc, abundant in the subventricular zone (SVZ) of the lateral ventricles. At 17.5 dpc and P0, expression is limited to distinct cells in the cortical SVZ, in cells of the secondary matrix, the dentate gyrus migratory route and the dentate gyrus.
Post Translational Modifications
Function DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). Mediates interstrand cross-link repair in response to replication stress: acts by mediating DNA glycosylase activity, cleaving one of the two N-glycosyl bonds comprising the interstrand cross-link, which avoids the formation of a double-strand break but generates an abasic site that is bypassed by translesion synthesis polymerases. In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA.also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues.
Protein Name Endonuclease 8-Like 3
Dna Glycosylase Fpg2
Dna Glycosylase/Ap Lyase Neil3
Endonuclease Viii-Like 3
Nei-Like Protein 3
Database Links Reactome: R-MMU-110331
Cellular Localisation Nucleus
Chromosome
Recruited To Replication Stress Sites Via Interaction With Ubiquitinated Cmg Helicase
Alternative ELISA Names Endonuclease 8-Like 3 ELISA kit
Dna Glycosylase Fpg2 ELISA kit
Dna Glycosylase/Ap Lyase Neil3 ELISA kit
Endonuclease Viii-Like 3 ELISA kit
Nei-Like Protein 3 ELISA kit
Neil3 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance