Mouse MMP-8 (Matrix Metalloproteinase 8) Sandwich ELISA (STJE0019732)

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STJE0019732

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16-10pg/mL
Short Description: This MMP-8 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Mmp8
Gene ID: 17394
Uniprot ID: MMP8_MOUSE
Specificity: This kit recognizes Mouse MMP-8 in samples. No significant cross-reactivity or interference between Mouse MMP-8 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Can degrade fibrillar type I, II, and III collagens. May play a role in the degradation of collagen fibers during uterine involution.
Protein Name Neutrophil Collagenase
Collagenase 2
Matrix Metalloproteinase-8
Mmp-8
Database Links Reactome: R-MMU-1442490
Reactome: -MMU-1474228
Reactome: -MMU-1592389
Reactome: -MMU-6798695
Cellular Localisation Cytoplasmic Granule
Secreted
Extracellular Space
Extracellular Matrix
Stored In Intracellular Granules And Released During Inflammatory Conditions
Alternative ELISA Names Neutrophil Collagenase ELISA kit
Collagenase 2 ELISA kit
Matrix Metalloproteinase-8 ELISA kit
Mmp-8 ELISA kit
Mmp8 ELISA kit
Specificity This kit recognizes Mouse MMP-8 in samples. No significant cross-reactivity or interference between Mouse MMP-8 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (50×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (50×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-110 101
EDTA plasma (n=8) 96-110 102
Cell culture media (n=8) 85-98 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.53 0.98 4.34 0.56 0.90 4.58
Standard deviation 0.03 0.05 0.14 0.04 0.05 0.16
CV (%) 5.65 5.23 3.25 6.26 5.41 3.60
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance