Applications: |
ELISA |
Reactivity: |
Mouse |
Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Sensitivity: |
0.052ng/mL |
Detection Limit: |
0.156-10ng/mL |
Short Description: |
This MID1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
Assay Time: |
3 hrs |
Gene Symbol: |
Mid1 |
Gene ID: |
17318 |
Uniprot ID: |
TRI18_MOUSE |
Immunogen Region: |
Ready-To-Use |
Sample Type: |
tissue homogenates or other biological fluids. |
Tissue Specificity | Ubiquitously expressed in fetus and adult. At 9 dpc-10.5 dpc, highest expression found in frontonasal processes, branchial arches and CNS. From 12.5 dpc to 16.5 dpc, high levels found in rostral part of CNS. At 14.5 dpc, begins to be highly expressed in kidney and lung. At 16.5 dpc, highly expressed in the mucosa of the hindgut and cutaneous region of the stomach. |
Post Translational Modifications | Phosphorylated. |
Function | Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination. |
Protein Name | E3 Ubiquitin-Protein Ligase Midline-1MidinRing Finger Protein Midline-1Ring-Type E3 Ubiquitin Transferase Midline-1Tripartite Motif-Containing Protein 18 |
Database Links | |
Cellular Localisation | CytoplasmCytoskeletonMicrotubule-Associated |
Alternative ELISA Names | E3 Ubiquitin-Protein Ligase Midline-1 ELISA kitMidin ELISA kitRing Finger Protein Midline-1 ELISA kitRing-Type E3 Ubiquitin Transferase Midline-1 ELISA kitTripartite Motif-Containing Protein 18 ELISA kitMid1 ELISA kitFxy ELISA kitTrim18 ELISA kit |
output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance