| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
5.46 pg/mL |
| Detection Limit: |
31.25-2000 pg/mL |
| Short Description: |
This MFGE8 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Mfge8 |
| Gene ID: |
17304 |
| Uniprot ID: |
MFGM_MOUSE |
| Specificity: |
This kit recognizes Mouse MFGE8 in samples. No significant cross-reactivity or interference between Mouse MFGE8 and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Tissue Specificity | Mammary epithelial cell surfaces and spermatozoan. Isoform 2 is present in brain, heart, kidney and spleen and at low levels in lung, liver, small intestine and testis. |
| Post Translational Modifications | N-glycosylated. Isoform 1 also exists in both an O-glycosylated and a non-O-glycosylated form. |
| Function | Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization. |
| Protein Name | LactadherinMfgmMilk Fat Globule-Egf Factor 8Mfg-E8Sed1Sperm Surface Protein Sp47Mp47 |
| Database Links | Reactome: R-MMU-381426Reactome: -MMU-8957275 |
| Cellular Localisation | MembranePeripheral Membrane ProteinSecretedCytoplasmic VesicleSecretory VesicleAcrosome MembraneLocated In The Acrosomal Region Of Zona-Pellucida Bound Sperm |
| Alternative ELISA Names | Lactadherin ELISA kitMfgm ELISA kitMilk Fat Globule-Egf Factor 8 ELISA kitMfg-E8 ELISA kitSed1 ELISA kitSperm Surface Protein Sp47 ELISA kitMp47 ELISA kitMfge8 ELISA kit |
| Specificity | This kit recognizes Mouse MFGE8 in samples. No significant cross-reactivity or interference between Mouse MFGE8 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-109 | 100 |
| EDTA plasma (n=8) | 89-101 | 95 |
| Cell culture media (n=8) | 90-108 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 109.70 | 203.71 | 814.03 | 110.75 | 211.69 | 784.39 |
| Standard deviation | 6.13 | 11.35 | 30.85 | 6.09 | 12.07 | 33.10 |
| CV (%) | 5.59 | 5.57 | 3.79 | 5.50 | 5.70 | 4.22 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
