Mouse MCP-1 (Monocyte Chemotactic Protein 1) Sandwich ELISA (STJE0019726)

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STJE0019726

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63-1000pg/mL
Short Description: This MCP-1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Ccl2
Gene ID: 20296
Uniprot ID: CCL2_MOUSE
Specificity: This kit recognizes Mouse MCP-1 in samples. No significant cross-reactivity or interference between Mouse MCP-1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B.
Protein Name C-C Motif Chemokine 2
Monocyte Chemoattractant Protein 1
Monocyte Chemotactic Protein 1
Mcp-1
Platelet-Derived Growth Factor-Inducible Protein Je
Small-Inducible Cytokine A2
Cellular Localisation Secreted
Alternative ELISA Names C-C Motif Chemokine 2 ELISA kit
Monocyte Chemoattractant Protein 1 ELISA kit
Monocyte Chemotactic Protein 1 ELISA kit
Mcp-1 ELISA kit
Platelet-Derived Growth Factor-Inducible Protein Je ELISA kit
Small-Inducible Cytokine A2 ELISA kit
Ccl2 ELISA kit
Je ELISA kit
Mcp1 ELISA kit
Scya2 ELISA kit
Specificity This kit recognizes Mouse MCP-1 in samples. No significant cross-reactivity or interference between Mouse MCP-1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (50×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (50×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-108 99
EDTA plasma (n=8) 86-101 93
Cell culture media (n=8) 93-107 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 53.10 142.67 493.35 55.18 141.85 506.70
Standard deviation 3.69 8.12 25.85 3.82 8.51 21.18
CV (%) 6.95 5.69 5.24 6.92 6.00 4.18
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance