| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63-1000pg/mL |
| Short Description: |
This MCP-1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Ccl2 |
| Gene ID: |
20296 |
| Uniprot ID: |
CCL2_MOUSE |
| Specificity: |
This kit recognizes Mouse MCP-1 in samples. No significant cross-reactivity or interference between Mouse MCP-1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B. |
| Protein Name | C-C Motif Chemokine 2Monocyte Chemoattractant Protein 1Monocyte Chemotactic Protein 1Mcp-1Platelet-Derived Growth Factor-Inducible Protein JeSmall-Inducible Cytokine A2 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | C-C Motif Chemokine 2 ELISA kitMonocyte Chemoattractant Protein 1 ELISA kitMonocyte Chemotactic Protein 1 ELISA kitMcp-1 ELISA kitPlatelet-Derived Growth Factor-Inducible Protein Je ELISA kitSmall-Inducible Cytokine A2 ELISA kitCcl2 ELISA kitJe ELISA kitMcp1 ELISA kitScya2 ELISA kit |
| Specificity | This kit recognizes Mouse MCP-1 in samples. No significant cross-reactivity or interference between Mouse MCP-1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-108 | 99 |
| EDTA plasma (n=8) | 86-101 | 93 |
| Cell culture media (n=8) | 93-107 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 53.10 | 142.67 | 493.35 | 55.18 | 141.85 | 506.70 |
| Standard deviation | 3.69 | 8.12 | 25.85 | 3.82 | 8.51 | 21.18 |
| CV (%) | 6.95 | 5.69 | 5.24 | 6.92 | 6.00 | 4.18 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
