• Mouse LOXL2 (Lysyl Oxidase Like Protein 2) Sandwich ELISA Kit (STJE0010386)

Mouse LOXL2 (Lysyl Oxidase Like Protein 2) Sandwich ELISA Kit (STJE0010386)

SKU:
STJE0010386

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Applications: ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.061ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This LOXL2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: Loxl2
Gene ID: 94352
Uniprot ID: LOXL2_MOUSE
Sample Type: serum, plasma, tissue homogenates or other biological fluids.
Tissue Specificity Ubiquitous. Highest expression in skin, lung and thymus. Present in chondrocytes: mainly expressed by chondrocytes in healing fractures and in epiphyseal growth plates (at protein level).
Post Translational Modifications The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine. N-glycosylated. N-glycosylation on Asn-458 and Asn-646 may be essential for proper folding and secretion.may be composed of a fucosylated carbohydrates attached to a trimannose N-linked glycan core.
Function Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). Acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Shows no activity against histone H3 when it is trimethylated on 'Lys-9' (H3K9me3) or 'Lys-27' (H3K27me3) or when 'Lys-4' is monomethylated (H3K4me1) or dimethylated (H3K4me2). Also mediates deamination of methylated TAF10, a member of the transcription factor IID (TFIID) complex, which induces release of TAF10 from promoters, leading to inhibition of TFIID-dependent transcription. LOXL2-mediated deamination of TAF10 results in transcriptional repression of genes required for embryonic stem cell pluripotency including POU5F1/OCT4, NANOG, KLF4 and SOX2. Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E-cadherin, probably by mediating deamination of histone H3. During EMT, involved with SNAI1 in negatively regulating pericentromeric heterochromatin transcription. SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits. Interacts with the endoplasmic reticulum protein HSPA5 which activates the IRE1-XBP1 pathway of the unfolded protein response, leading to expression of several transcription factors involved in EMT and subsequent EMT induction. When secreted into the extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation.
Protein Name Lysyl Oxidase Homolog 2
Lysyl Oxidase-Like Protein 2
Database Links Reactome: R-MMU-1566948
Reactome: -MMU-2243919
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Basement Membrane
Nucleus
Chromosome
Endoplasmic Reticulum
Associated With Chromatin
It Is Unclear How Loxl2 Is Nuclear As It Contains A Signal Sequence And Has Been Shown To Be Secreted
However
A Number Of Reports Confirm Its Intracellular Location And Its Key Role In Transcription Regulation
Alternative ELISA Names Lysyl Oxidase Homolog 2 ELISA kit
Lysyl Oxidase-Like Protein 2 ELISA kit
Loxl2 ELISA kit
output

Information sourced from Uniprot.org

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