• Mouse LIPD (Lipase, Lipoprotein) Sandwich ELISA Kit (STJE0010353)

Mouse LIPD (Lipase, Lipoprotein) Sandwich ELISA Kit (STJE0010353)

SKU:
STJE0010353

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Applications: ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 24pg/mL
Detection Limit: 78.1-5000pg/mL
Short Description: This LIPD Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: Lpl
Gene ID: 16956
Uniprot ID: LIPL_MOUSE
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Tissue Specificity Detected in white and brown adipose tissue and heart muscle, especially at the lumenal surface of capillaries. Detected on capillary endothelium in the lactating mammary gland. Detected in blood plasma (at protein level). Expressed in liver, epididymal fat, heart, psoas muscle, lactating mammary gland, adrenal, lung, and ovary. Highest levels in heart and adrenal gland.
Post Translational Modifications Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity. N-glycosylated.
Function Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage. Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity. Mediates margination of triglyceride-rich lipoprotein particles in capillaries. Recruited to its site of action on vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans.
Protein Name Lipoprotein Lipase
Lpl
Phospholipase A1
Database Links Reactome: R-MMU-8963889
Reactome: -MMU-8963901
Reactome: -MMU-975634
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Extracellular Side
Secreted
Extracellular Space
Extracellular Matrix
Newly Synthesized Lpl Binds To Cell Surface Heparan Proteoglycans And Is Then Released By Heparanase
Subsequently
It Becomes Attached To Heparan Proteoglycan On Endothelial Cells
Locates To The Plasma Membrane Of Microvilli Of Hepatocytes With Triglyceride-Rich Lipoproteins (Trl)
Some Of The Bound Lpl Is Then Internalized And Located Inside Non-Coated Endocytic Vesicles
Alternative ELISA Names Lipoprotein Lipase ELISA kit
Lpl ELISA kit
Phospholipase A1 ELISA kit
Lpl ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance