| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.1ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This LIPA Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Lipa |
| Gene ID: |
16889 |
| Uniprot ID: |
LICH_MOUSE |
| Specificity: |
This kit recognizes Mouse LIPA in samples. No significant crossreactivity or interference between Mouse LIPA and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Tissue Specificity | Expressed at low levels in most tissues. High level expression is found in hepatocytes and splenic and thymic cells. Very high level expression is observed in cells of the small intestinal villi, the zona fasciculata and reticularis of the adrenal cortex, pancreatic acini, and renal tubular epithelium. |
| Post Translational Modifications | Glycosylation is not essential for catalytic activity. |
| Function | Catalyzes the deacylation of triacylglyceryl and cholesteryl ester core lipids of endocytosed low density lipoproteins to generate free fatty acids and cholesterol. |
| Protein Name | Lysosomal Acid Lipase/Cholesteryl Ester HydrolaseAcid Cholesteryl Ester HydrolaseLalCholesteryl EsteraseLipase ASterol Esterase |
| Database Links | Reactome: R-MMU-8964038 |
| Cellular Localisation | Lysosome |
| Alternative ELISA Names | Lysosomal Acid Lipase/Cholesteryl Ester Hydrolase ELISA kitAcid Cholesteryl Ester Hydrolase ELISA kitLal ELISA kitCholesteryl Esterase ELISA kitLipase A ELISA kitSterol Esterase ELISA kitLipa ELISA kitLip1 ELISA kit |
| Specificity | This kit recognizes Mouse LIPA in samples. No significant cross-reactivity or interference between Mouse LIPA and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-109 | 101 |
| EDTA plasma (n=8) | 95-108 | 101 |
| Cell culture media (n=8) | 85-101 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.54 | 1.54 | 3.69 | 0.52 | 1.48 | 3.78 |
| Standard deviation | 0.03 | 0.07 | 0.16 | 0.03 | 0.08 | 0.13 |
| CV (%) | 5.56 | 4.55 | 4.34 | 5.77 | 5.41 | 3.44 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
