| Host: |
HEK293 cells |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Mouse Latent TGF-beta 2-N-8*His protein was developed from hek293 cells and has a target region of N-8*His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: |
Measured by its ability to inhibit the IL-4 (Catalog: RP01161)-dependent proliferation of HT-2 mouse T cells. The ED50 for this effect is 2.31-9.23 ng/mL, corresponding to a specific activity of 1.08×105~4.33×105 units/mg. |
| Gene Symbol: |
Tgfb2 |
| Gene ID: |
21808 |
| Uniprot ID: |
TGFB2_MOUSE |
| Immunogen Region: |
Leu21-Ser414 |
| Immunogen: |
Recombinant Mouse Latent TGF-beta 2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu21-Ser414) of mouse Latent TGF-beta 2 (Accession #NP_033393.2) fused with a 8×His tag at the N-terminus. |
| Function | Transforming growth factor beta-2 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively. Latency-associated peptide: Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2. Transforming growth factor beta-2: Multifunctional protein that regulates various processes such as angiogenesis and heart development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus. Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal. |
| Protein Name | Transforming Growth Factor Beta-2 Proprotein Cleaved Into - Latency-Associated PeptideLap - Transforming Growth Factor Beta-2Tgf-Beta-2 |
| Database Links | Reactome: R-MMU-114608Reactome: -MMU-2129379Reactome: -MMU-2173789 |
| Cellular Localisation | Latency-Associated Peptide: SecretedExtracellular SpaceExtracellular MatrixTransforming Growth Factor Beta-2: Secreted |
| Alternative Protein Names | Transforming Growth Factor Beta-2 Proprotein Cleaved Into - Latency-Associated Peptide proteinLap - Transforming Growth Factor Beta-2 proteinTgf-Beta-2 proteinTgfb2 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
