| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This Itgam Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
ItgamUniprot ID=ITAM_MOUSE" |
| Specificity: |
This kit recognizes Mouse Itgam/CD11b in samples. No significant cross-reactivity or interference between Mouse Itgam/CD11b and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils. May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development. |
| Protein Name | Integrin Alpha-MCd11 Antigen-Like Family Member BCr-3 Alpha ChainCell Surface Glycoprotein Mac-1 Subunit AlphaLeukocyte Adhesion Receptor Mo1Cd Antigen Cd11b |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane Raft |
| Alternative ELISA Names | Integrin Alpha-M ELISA kitCd11 Antigen-Like Family Member B ELISA kitCr-3 Alpha Chain ELISA kitCell Surface Glycoprotein Mac-1 Subunit Alpha ELISA kitLeukocyte Adhesion Receptor Mo1 ELISA kitCd Antigen Cd11b ELISA kitItgam ELISA kit |
| Specificity | This kit recognizes Mouse Itgam/CD11b in samples. No significant cross-reactivity or interference between Mouse Itgam/CD11b and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 84-98 | 90 |
| EDTA plasma (n=8) | 91-103 | 98 |
| Cell culture media (n=8) | 94-108 | 102 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 52.06 | 108.27 | 454.33 | 53.28 | 104.62 | 465.14 |
| Standard deviation | 3.57 | 6.18 | 14.81 | 2.66 | 5.34 | 25.30 |
| CV (%) | 6.86 | 5.71 | 3.26 | 4.99 | 5.10 | 5.44 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
